A total of six human EC cells (Ishikawa, AN3 CA, HEC-1-A, SPEC-2, RL95-2 and KLE) were purchased from the American Type Culture Collection (ATCC; Rockville, MD, US). Fetal bovine serum (FBS), Dulbecco's modified Eagle's medium (DMEM)/high glucose medium, minimal essential medium (MEM), McCoy's 5A medium and DMEM/F12 medium were purchased from Gibco, Thermo Fisher Scientific, Inc. (Waltham, MA, USA). Anti-FOXO1 antibodies (no. ab39670) were purchased from Abcam (Cambridge, UK), anti-SREBP1 antibodies (H-160; no. sc-8984) from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA) and anti-GAPDH antibodies (no. G9545) from Sigma-Aldrich; Merck Millipore (Darmstadt, Germany).
Dulbecco s modified eagle s medium dmem high glucose medium
Manufactured by Thermo Fisher Scientific
Sourced in United States
Dulbecco's modified Eagle's medium (DMEM)/high glucose medium is a cell culture medium that provides nutrients and support for the growth and maintenance of various cell types. It contains a high concentration of glucose, which serves as an energy source for the cells. The medium is designed to maintain the pH, osmolarity, and other essential components required for optimal cell growth and viability.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Dmem f12 medium, by Thermo Fisher Scientific (1 mentions)
Hec 1a, by American Type Culture Collection (1 mentions)
Ishikawa, by American Type Culture Collection (1 mentions)
An3ca, by American Type Culture Collection (1 mentions)
Rl95 2, by American Type Culture Collection (1 mentions)
Spec 2, by American Type Culture Collection (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 donkey anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 568 donkey anti mouse igg h l, by Thermo Fisher Scientific (1 mentions)
Sarcomeric α actinin, by Abcam (1 mentions)
α smooth muscle actin α sma, by Abcam (1 mentions)
2 protocols using dulbecco s modified eagle s medium dmem high glucose medium
1
Establishment and Characterization of EC Cell Lines
2
Reagents for Cell Culture and Immunohistochemistry
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Py was obtained from Sigma-Aldrich Corporation (USA). Other chemical reagents were obtained from Guanghua Chemical Factory (China). Dulbecco’s modified Eagle’s medium (DMEM)/high-glucose medium, fetal bovine serum (FBS), and trypsin were purchased from Gibco (USA). The primary antibodies including CD68, Ki-67, vWF, α-smooth muscle actin (α-SMA), sarcomeric α-actinin, and CX-43 were ordered from Abcam (Britain). Alexa Fluor 488 Donkey Anti-Rabbit IgG (H+L) and Alexa Fluor 568 Donkey Anti-Mouse IgG (H+L) were procured from Life Technologies (USA).
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