To transiently inhibit miR-21 expression, hsa-miR-21 mirVana™ miRNA inhibitor (Applied Biosystems) or hsa-miR-21 mirVana® mimics (Applied Biosystems) were used to transfect HNSCC cells, as described previously [19 (link)]. Verification of transfection efficiency was conducted using the mirVana™ miRNA Mimic Negative Control (Applied Biosystems) and mirVana™ miRNA Inhibitor Negative Control (Applied Biosystems), respectively. Forward transfections were conducted by mixing miRNA mimic or inhibitor (70 nM) with Lipofectamine RNAiMAX (Invitrogen, Carlsbad, CA, USA) and Opti-MEM I (Invitrogen) and applying the mixture to cells at 24 h after plating. A series of in vitro assays was conducted after 48–72 h of incubation.
Hsa mir 21 mirvana mimics
Manufactured by Thermo Fisher Scientific
The Hsa-miR-21 mirVana® mimics are a laboratory product designed to facilitate the study of the microRNA hsa-miR-21. The product contains synthetic RNA molecules that mimic the sequence and function of the naturally occurring hsa-miR-21 microRNA. The core function of this product is to provide a tool for researchers to investigate the biological roles and mechanisms of hsa-miR-21 in various experimental systems.
Automatically generated - may contain errors
2 protocols using hsa mir 21 mirvana mimics
1
Transient miR-21 Inhibition in HNSCC
2
Transient Inhibition of miR-21 in HNSCC
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To transiently inhibit miR-21 expression, hsa-miR-21 mirVana™ miRNA inhibitor (Applied Biosystems) or hsa-miR-21 mirVana® mimics (Applied Biosystems) were used to transfect HNSCC cells, as described previously [18] . Veri cation of transfection e ciency was conducted using the mirVana™ miRNA Mimic Negative Control (Applied Biosystems) and mirVana™ miRNA Inhibitor Negative Control (Applied Biosystems), respectively. Forward transfections were conducted by mixing siRNA oligonucleotides (70 nM) with Lipofectamine RNAiMAX (Invitrogen, Carlsbad, CA, USA) and Opti-MEM I (Invitrogen) and applying the mixture to cells at 24 h after plating. A series of in vitro assays was conducted after 48-72 h of incubation.
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