Lysis buffer containing 50 mmol/L Tris-HCl, 150 mmol/L NaCl, 1% NP 40, 0.1% SDS, and 0.5% deoxycholate and Protease Inhibitor Cocktail (Roche, Indianapolis, IN, USA) was used to prepare all cell protein lysates. Total protein lysates (25–30 μg) were electrophoresed in SDS–polyacrylamide gel and immunoblotted with an anti-EDNRA antibody (1:200; Santa Cruz Biotechnology, Santa Cruz, CA, USA) or an anti-CDH2 antibody (1:1,000; Santa Cruz Biotechnology). A primary antibody against α-tubulin (1:5,000; Sigma) was used as an internal control.
Primary antibody against α tubulin
Manufactured by Merck Group
The primary antibody against α-tubulin is a laboratory reagent used for the detection and localization of α-tubulin, a key structural component of microtubules in eukaryotic cells. This antibody can be used in various immunochemical techniques, such as Western blotting, immunofluorescence, and immunohistochemistry, to study the distribution and dynamics of microtubules within cells.
Automatically generated - may contain errors
Lab products found in correlation
Protease inhibitor cocktail, by Roche (1 mentions)
P enos, by Cell Signaling Technology (1 mentions)
Tunel assay kit, by Promega (1 mentions)
Cox 2, by Cell Signaling Technology (1 mentions)
Akap12, by Abcam (1 mentions)
Vascular endothelial growth factor (vegf), by Abcam (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
2 protocols using primary antibody against α tubulin
1
Western Blot Analysis of EDNRA and CDH2
2
Comprehensive Antibody Sourcing Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Primary antibody against α-Tubulin was purchased from Sigma-Aldrich (St Louis, MO). The primary antibody against HSPA12B was a generous gift from Dr. Zhihua Han (East Tennessee State University) [9 ]. The remaining primary antibodies and the companies that supplied them were as follows: Bcl-2, Bax, Cox-2 and p-eNOS (Cell Signaling Technology, Beverly, MA); PCAM-1 and Ki-67 (BD Pharmingen, San Jose, CA); eNOS (BD Biosciences, Bedford, MA); and VEGF, Ang-1 and AKAP12 (Abcam, Cambridge, UK). Celecoxib was obtained from Pfizer Pharmaceuticals LLC (Barceloneta, PR). The TUNEL assay kit was from Promega (Madison, WI). The supersignal west pico chemiluminescent substrate was obtained from Pierce (Rockford, IL).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!