Mouse anti dsrna j2 clone

Manufactured by Techcomp Instruments

The Mouse anti-dsRNA (J2 clone) is a monoclonal antibody that specifically recognizes double-stranded RNA (dsRNA). It serves as a tool for the detection and analysis of dsRNA in various applications.

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Lab products found in correlation

Goat anti mouse af488, by Thermo Fisher Scientific (1 mentions) Mannan from saccharomyces cerevisiae, by Merck Group (1 mentions) Donkey anti rabbit af647, by Thermo Fisher Scientific (1 mentions) Donkey anti mouse af568, by Thermo Fisher Scientific (1 mentions) Facsymphony a3 cell analyzer, by BD (1 mentions) Cytoperm fix kit, by BD (1 mentions) Anti rabbit igg pacific blue, by BD (1 mentions)

Spelling variants of this product

Anti-dsRNA (J2; (9 citations) Mouse J2 anti-dsRNA (6 citations) DsRNA-J2 (4 citations) Mouse anti-dsRNA (4 citations) DsRNA (3 citations)

2 protocols using mouse anti dsrna j2 clone

Antibody Labeling and Mannan Detection

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The primary antibodies used were mouse anti-dsRNA (J2 clone, Scicons), mouse pan-flavivirus (4G2 clone, Novus Biologicals), rabbit anti-MX1 (Thermo Fischer Scientific), mouse APC conjugated anti-langerin (clone 10E2, BioLegend), rabbit anti-langerin (clone D9H7R, Cell Signaling), recombinant anti-DC-SIGN (clone REA617, Miltenyi Biotec), mouse anti-CD1a (Novus Biologicals), FITC-conjugated anti-CD1a (clone HI149, Miltenyi Biotec), FITC-conjugated anti-HLA-DR (clone AC122, Miltenyi Biotec), mouse anti-HSP90 (clone F-8, Santa Cruz), and mouse anti-GAPDH (clone 6C5, Merck Millipore). Secondary antibodies were goat anti-mouse AF488 (Thermo Fisher Scientific), donkey anti-mouse AF568 (Thermo Fisher Scientific), donkey anti-rabbit AF647 (Thermo Fisher Scientific) and goat anti-mouse or anti-rabbit HRP conjugates (GE Healthcare).
Mannan from Saccharomyces cerevisiae was purchased from Sigma-Aldrich.

Martin M.F., Maarifi G., Abiven H., Seffals M., Mouchet N., Beck C., Bodet C., Lévèque N., Arhel N.J., Blanchet F.P., Simonin Y, & Nisole S. (2022). Usutu Virus escapes langerin-induced restriction to productively infect human Langerhans cells, unlike West Nile virus. Emerging Microbes & Infections, 11(1), 761-774.

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Viral Infection and dsRNA Detection

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HeLa cells were mock- or virus-infected with either wt VACV, ΔE3, E3Δ37N, E3Δ37N^IBTR−3, E3Δ37N^IBTR-7, VACV-A24R-R1, or MPXV. At 6 hpi, cells were trypsinized and fixed with CytoPerm/Fix kit (BD). Cells were stained with rabbit anti-VACV (Abcam) and mouse anti-dsRNA (J2 clone, Scicons) overnight at 4°C. Cells were washed and stained with anti-rabbit-IgG Pacific Blue (BD) and anti-mouse-IgG 488 (Invirtogen) for 4 °C. Cells were washed, resuspended in FACS buffer (2% BSA, 0.9% sodium azide in PBS) and were analyzed by flow cytometry using a BD FACSymphony™ A3 Cell Analyzer and cells were gated to exclude doublet and non-viable cells.

Koehler H., Cotsmire S., Zhang T., Balachandran S., Upton J.W., Langland J., Kalman D., Jacobs B.L, & Mocarski E.S. (2021). Vaccinia virus E3 prevents sensing of Z-RNA to block ZBP1-dependent necroptosis. Cell host & microbe, 29(8), 1266-1276.e5.

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