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S75 10 300 increase gl

Manufactured by GE Healthcare
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The S75 10/300 Increase GL is a lab equipment product from GE Healthcare. It is designed for gel filtration chromatography applications. The device has a bed volume of 10 ml and a column size of 10/300 mm.

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Lab products found in correlation

Superdex 200 increase 10 300 gl, by GE Healthcare (2 mentions) Bira500, by Avidity (2 mentions)

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S75 10/300 GL

2 protocols using s75 10 300 increase gl

1

Biotinylation and Purification of Avi-tagged Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Avi-tagged (GLNDIFEAQKIEWHE, see supplement) proteins were purified as described above. The BirA500 (Avidity, LLC) biotinylation kit was used to biotinylate 840 uL of protein from the IMAC elution in a 1200 uL (final volume) reaction according to the manufacturer’ protocol. Reactions were incubated at 4 degrees C o/n and purified using size exclusion chromatography on a Superdex 200 10/300 Increase GL (GE Healthcare) or S75 10/300 Increase GL (GE Healthcare) in SEC buffer (20 mM Tris pH 8.0, 100 mM NaCl).
Sahtoe D.D., Praetorius F., Courbet A., Hsia Y., Wicky B.I., Edman N.I., Miller L.M., Timmermans B.J., Decarreau J., Morris H.M., Kang A., Bera A.K, & Baker D. (2022). Reconfigurable asymmetric protein assemblies through implicit negative design. Science (New York, N.Y.), 375(6578), eabj7662.
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2

Biotinylation and Purification of Avi-tagged Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Avi-tagged (GLNDIFEAQKIEWHE, see supplement) proteins were purified as described above. The BirA500 (Avidity, LLC) biotinylation kit was used to biotinylate 840 uL of protein from the IMAC elution in a 1200 uL (final volume) reaction according to the manufacturer’ protocol. Reactions were incubated at 4 degrees C o/n and purified using size exclusion chromatography on a Superdex 200 10/300 Increase GL (GE Healthcare) or S75 10/300 Increase GL (GE Healthcare) in SEC buffer (20 mM Tris pH 8.0, 100 mM NaCl).
Sahtoe D.D., Praetorius F., Courbet A., Hsia Y., Wicky B.I., Edman N.I., Miller L.M., Timmermans B.J., Decarreau J., Morris H.M., Kang A., Bera A.K, & Baker D. (2022). Reconfigurable asymmetric protein assemblies through implicit negative design. Science (New York, N.Y.), 375(6578), eabj7662.
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