Ibright cl1000

Manufactured by Merck Group

The IBright CL1000 is a compact and versatile lab equipment designed for a range of applications. It functions as a chemiluminescence imaging system, providing high-sensitivity detection and analysis of chemiluminescent samples. The device features a cooled CCD camera and advanced imaging software to capture and process chemiluminescent signals. The core function of the IBright CL1000 is to enable sensitive detection and quantification of chemiluminescent samples in a laboratory setting.

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Lab products found in correlation

Luminescent substrate, by Merck Group (2 mentions) Anti gapdh mab, by Thermo Fisher Scientific (2 mentions) Anti spike mab 1a9, by GeneTex (1 mentions) Hrp conjugated goat anti mouse secondary antibody, by Merck Group (1 mentions) Goat anti mouse hrp conjugated second antibody, by Merck Group (1 mentions) Mouse anti his mab, by Thermo Fisher Scientific (1 mentions) Anti ha mab, by Fortrea (1 mentions)

2 protocols using ibright cl1000

SARS-CoV-2 Spike Protein Analysis

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Spike proteins were analyzed on immunoblots probed with transfected cells that were lysed in buffer containing 50 mM HEPES, 150 mM KCl, 2 mM EDTA, 0.5% NP-40, and protease inhibitor cocktail. Protein (40 μg) was separated by SDS-PAGE. The proteins were transferred to polyvinylidene difluoride membranes and probed with anti-spike mAb (1A9) (GeneTex), anti-p24 mAb (AG3.0) and anti-GAPDH mAb (Life Technologies) followed by goat anti-mouse HRP-conjugated secondary antibody (Sigma). The proteins were visualized by luminescent substrate (Millipore) on iBright CL1000.

Tada T., Zhou H., Dcosta B.M., Samanovic M.I., Mulligan M.J, & Landau N.R. (2021). Partial resistance of SARS-CoV-2 Delta variants to vaccine-elicited antibodies and convalescent sera. iScience, 24(11), 103341.

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Western Blot Analysis of Transfected Cells

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Transfected cells were lysed in buffer containing 50 mM HEPES, 150 mM KCl, 2 mM EDTA, 0.5% NP-40, and protease inhibitor cocktail. Protein concentration in the lysates was measured by bicinchoninic protein assay and the lysates (40 μg) were separated by SDS-PAGE. The proteins were transferred to polyvinylidene difluoride membranes and probed with anti-HA mAb (Covance), mouse anti-His mAb (Invitrogen) and anti-GAPDH mAb (Life Technologies) followed by goat anti-mouse HRP-conjugated second antibody (Sigma). The blots were visualized using luminescent substrate (Millipore) on iBright CL1000.

Tada T., Fan C., Chen J.S., Kaur R., Stapleford K.A., Gristick H., Dcosta B.M., Wilen C.B., Nimigean C.M, & Landau N.R. (2020). An ACE2 Microbody Containing a Single Immunoglobulin Fc Domain Is a Potent Inhibitor of SARS-CoV-2. Cell Reports, 33(12), 108528.

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