Clinical laboratory studies were done in compliance with USAMRIID’s Clinical Laboratory procedures. Hematology was performed using an AcT10 Hematology Analyzer by Beckman Coulter, and urinalysis was done utilizing a Multistix 10 SG, by Siemens. Clinical chemistries were performed using a Piccolo Point of Care General Chemistry panel (ALB, ALP, ALT, AMY, AST, BUN, Ca, CRE, GGT, GLU, TBIL, TP) by Abaxis. HIV Ab were done at USAMRIID using the Clinical Laboratory’s routine HIV testing kit, Bio-Rad Laboratories Multispot HIV-1/HIV-2 Rapid Test. Throat swabs were done using routine daily clinical methodology.
Act10 hematology analyzer
Manufactured by Beckman Coulter
The AcT10 Hematology Analyzer is a fully automated instrument designed for the analysis of complete blood count (CBC) and other related hematology parameters. It utilizes advanced technology to provide accurate and reliable results.
Automatically generated - may contain errors
2 protocols using act10 hematology analyzer
1
Clinical Laboratory Studies Methodology
2
Isolation of Murine Immune Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Thymus, spleen, and lymphonode were collected from 4–12-week-old mice and minced into small pieces in the presence of HBSS (Lonza, Allendale, NJ) supplied with 2% FBS. A single cell suspension was prepared using 40 μm cell strainer (BD, San Jose, CA). Excessive red blood cells were removed by incubation with ACK lysing buffer (Lonza) per manufacturer's instruction. The peripheral blood was sampled from tail vain bleeding and peripheral blood mononuclear cells were prepared using ACK lysing buffer. All cells were washed with HBSS before staining for flow cytometry analysis. Cellularity was counted using AcT10 hematology analyzer (Beckman Coulter, Miami, FL).
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