The apoptotic incidence was evaluated by the Annexin V-phycoerythrin (PE)/propidium iodide (PI) apoptosis detection kit (BD Biosciences, Franklin Lakes, NJ, USA) according to the manufacturer's instructions. Briefly, treated chondrocytes were collected and washed by PBS buffer, then incubated with 5 µl Annexin V-PE and 5 µl PI for 30 min at room temperature in the dark. Finally, Ending buffer was added, and cell apoptotic rate was analyzed using a flow cytometer (BD Biosciences).
Annexin 5 phycoerythrin pe propidium iodide apoptosis detection kit
Manufactured by BD
Sourced in United States, China
The Annexin V-phycoerythrin (PE)/propidium iodide (PI) apoptosis detection kit is a laboratory instrument used to identify and quantify apoptotic cells. It employs the binding of Annexin V-PE to exposed phosphatidylserine, and the detection of membrane integrity using PI, to differentiate between viable, early apoptotic, and late apoptotic/necrotic cells.
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Lab products found in correlation
2 protocols using annexin 5 phycoerythrin pe propidium iodide apoptosis detection kit
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Annexin V-PE/PI Apoptosis Assay
2
Chondrocyte Isolation and Characterization
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Pentobarbital sodium was purchased from Merck KGaA (Darmstadt, Germany). Safranin O-Fast Green staining reagent was obtained from Beijing Leagene Biotech Co., Ltd. (Beijing, China). Dulbecco's modified Eagle's medium (DMEM)/F12 and fetal bovine serum were obtained from HyClone; GE Healthcare Life Sciences (Logan, UT, USA). Recombinant Human LIF Protein was purchased from Novus Biologicals, LLC (Littleton, CO, USA). Collagenase type II was obtained from Sigma-Aldrich; Merck KGaA. Collagen type II α1 (COL2α1) and LIF primary antibodies were from BIOSS (Beijing, China; bs-1068R and bs-1058R), and aggrecan primary antibody was from Abcam (Cambridge, MA, USA; ab3778). Cell Counting Kit-8 (CCK-8) was purchased from Beyotime Institute of Biotechnology (Haimen, China) and the Annexin V-phycoerythrin (PE)/propidium iodide (PI) apoptosis detection kit was obtained from BD Biosciences (Franklin Lakes, NJ, USA).
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