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Probiotic Formulation Characterization and Evaluation

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The caffeic acid, keracyanin chloride, kuromanin chloride, Folin-Ciocalteau, and quercetin used in the experiment were purchased from Sigma-Aldrich (St. Louis, MO, USA). Aluminum chloride hexahydrate and potassium acetate were purchased from Junsei Chemical (Tokyo, Japan). Citric acid, sodium alginate, mannitol, and poloxamer 188 were purchased from Daejung (Seoul, Republic of Korea). Whey protein isolate, lecithin, and ascorbyl palmitate were purchased from ESfood (Gumpo, Republic of Korea). Lacticaseibacillus rhamnosus (L. rhamnosis), Pediococcus pentosaceus (P. pentosaceus), Enterococcus faecalis (E. faecalis). Escherichia coli (E. coli), and Streptococcus aureus (E. aureus) were purchased from ATCC (American Type Culture Collection). Man-Rogasa-shape (MRS) and brain heart infusion (BHI) medium was purchased from Solarbio (Shanghai, China).
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2

Sinorhizobium meliloti 1021 Mutagenesis

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The bacterial strain (Sinorhizobium meliloti 1021) was supplied by the Microbial Carbohydrate Resource Bank (MCRB) at Konkuk University (Seoul, Republic of Korea). Mannitol, glutamic acid, potassium phosphate monobasic, potassium phosphate dibasic, magnesium sulfate, and calcium chloride were purchased from Daejung (Busan, Republic of Korea). N-methyl-N′-nitro-N-nitrosoguanidine, sodium thiosulfate, Tris-Maleate, xanthan, alginate, and pullulan were purchased from Sigma-Aldrich (Steinheim, Germany).
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