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Hacat cell line

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The HaCaT cell line is a spontaneously immortalized human keratinocyte cell line. It is derived from normal human skin and is commonly used as an in vitro model for skin-related research and applications.

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Lab products found in correlation

Potassium phosphate, by Merck Group (1 mentions) Sodium dihydrogen phosphate monohydrate, by Merck Group (1 mentions) Magnesium sulfate heptahydrate, by Merck Group (1 mentions) Sodium ammonium phosphate tetrahydrate, by Merck Group (1 mentions) Magnesium chloride hexahydrate, by Merck Group (1 mentions) Muller hinton agar, by Thermo Fisher Scientific (1 mentions) Muller hinton broth, by Thermo Fisher Scientific (1 mentions) 4 nitro o phenylenediamine, by Merck Group (1 mentions) D biotin, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Sartorius (1 mentions) Dulbecco s modified eagle medium dmem, by Merck Group (1 mentions) Potassium chloride (kcl), by Merck Group (1 mentions) Sodium chloride (nacl), by Merck Group (1 mentions) Citric acid monohydrate, by Merck Group (1 mentions) Disodium hydrogen phosphate dihydrate, by Merck Group (1 mentions) Sodium azide, by Merck Group (1 mentions) 3t3 l1 cl 173, by American Type Culture Collection (1 mentions) 1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride edc, by Tokyo Chemical Industry (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions) 4 dimethylaminopyridine dmap, by Merck Group (1 mentions)

5 protocols using hacat cell line

1

Synthesis and Characterization of PLGA Nanoparticles

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PLGA, dichloromethane (DCM) and polyvinyl alcohol (PVA), citric acid monohydrate, sodium dihydrogen phosphate monohydrate, 4-nitro-o-phenylenediamine (NPD), magnesium sulfate heptahydrate, potassium phosphate, D-biotin, sodium chloride, magnesium chloride hexahydrate, disodium hydrogen phosphate dihydrate, potassium chloride, Nutrient Broth (No 2), L-histidine, Dulbecco’s Modified Eagle Medium (DMEM) and sodium ammonium phosphate tetrahydrate were purchased from Sigma–Aldrich (MO, USA). The O. acanthium extract (code: 5782ECH) was obtained from AZELIS TR KIMYA (Istanbul, Turkey). Agar was purchased from Difco (MD, USA). Muller-Hinton broth and Muller Hinton agar were purchased from Oxoid (Basingstoke, United Kingdom). Sodium azide was obtained from Merck (Darmstadt, Germany). HaCaT cell line was purchased from Thermo Fisher Scientific. 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) was purchased from Biomatik. Fetal Bovine Serum (FBS) and Penicillin-Streptomycin (10X) Solution were purchased from Biological Industries.
Budama-Kilinc Y., Gok B., Cetin Aluc C, & Kecel-Gunduz S. (2023). In vitro and in silico evaluation of the design of nano-phyto-drug candidate for oral use against Staphylococcus aureus. PeerJ, 11, e15523.
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2

Cell Line Characterization Protocol

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Cell lines 3T3-L1 (CL-173) and WS1 (CRL-1502) were both from the American Type Culture Collection (ATCC). The HaCaT cell line was obtained from Thermo Fisher Scientific.
Skóra M., Obłoza M., Tymecka M., Kalaska B., Gurgul M, & Kamiński K. (2023). Studies on Antifungal Properties of Methacrylamido Propyl Trimethyl Ammonium Chloride Polycations and Their Toxicity In Vitro. Microbiology Spectrum, 11(3), e00844-23.
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3

Culturing Immortalized Human Keratinocytes

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The immortalized human epidermal keratinocyte (HaCaT) cell line was obtained from Thermo Fisher Scientific (Waltham, MA, USA). The HaCaT cells were cultured in complete media, which comprised of Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum and 1% (v/v) penicillin-streptomycin at 37 °C in a humidified atmosphere of 5% CO2/95% air. All reagents were purchased from Invitrogen (Grand Island, NY, USA).
Warinhomhoun S., Muangnoi C., Buranasudja V., Mekboonsonglarp W., Rojsitthisak P., Likhitwitayawuid K, & Sritularak B. (2021). Antioxidant Activities and Protective Effects of Dendropachol, a New Bisbibenzyl Compound from Dendrobium pachyglossum, on Hydrogen Peroxide-Induced Oxidative Stress in HaCaT Keratinocytes. Antioxidants, 10(2), 252.
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4

Synthesis and Characterization of MPA-CUR Conjugate

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MPA (MW 320.3 g/mol) was obtained from AK Scientific (Union City, CA, USA), and CUR (MW 368.4 g/mol) was synthesized and characterized by the previously published method [32 (link)]. The 1-Ethyl-3-(3-dimethylamino propyl) carbodiimide hydrochloride (EDC) was obtained from Tokyo Chemical Industry (Tokyo, Japan). Moreover, 4-Dimethylaminopyridine (DMAP) was bought from Sigma Aldrich (St. Louis, MO, USA). HaCaT cell line was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Caco-2 cell line (ATCC No. HTB37) was obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Sigma Aldrich (St. Louis, MO, USA). Other materials, including solvents and chemicals, were purchased from commercial sources and used without further purification.
Yuyun Y., Ratnatilaka Na Bhuket P., Supasena W., Suwattananuruk P., Praengam K., Vajragupta O., Muangnoi C, & Rojsitthisak P. (2021). A Novel Curcumin-Mycophenolic Acid Conjugate Inhibited Hyperproliferation of Tumor Necrosis Factor-Alpha-Induced Human Keratinocyte Cells. Pharmaceutics, 13(7), 956.
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5

Investigating Cellular Stress Response

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TPP was bought from Merck. The HaCaT cell line was obtained from ThermoFisher. All of the cell culture materials were purchased from Gibco. The apoptosis/necrosis assay kit was obtained from Invitrogen. 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT), neutral red dye, dimethyl sulfoxide (DMSO) were from Biomatik. of 5 ′ , 6 ′ -chloromethyl-2 ′ ,7 ′ -dichlorofluorescein diacetate (H 2 DCFDA) dye and Bradford reagent were bought from Sigma-Aldrich. HSP90, HSP70, GRP94 and GRP78 antibodies were obtained from Cell Signaling. HRP conjugated anti-rabbit and anti-mouse, ubiquitin, GAPDH, HSP40 antibodies and RIPA buffer were from SCBT. Human Protein Carbonyl ELISA Kit was obtained from BT-Lab. Thiobarbituric acid (TBARS) Assay Kit was bought from RayBiotek. All other chemicals were from Sigma-Aldrich if not otherwise stated.
Jannuzzi A.T., Yilmaz Goler A.M, & Alpertunga B. (2022). Ubiquitin proteasomal system is a potential target of the toxic effects of organophosphorus flame retardant triphenyl phosphate. Environmental toxicology and pharmacology, 96.
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