DSPE-mPEG2000 was purchased from Xi’an Ruixi Biological Technology Co., Ltd. (China). 1,2-Dipalmitoyl-sn-glycerol-3-phosphocholine (DPPC), Cholesterol, PFP, IR780 were obtained from Sigma-Aldrich (St. Louis, MO, USA). Singlet Oxygen Sensor Green (SOSG) reagent, MitoTracker Green, and LysoTracker Green were obtained from Dalian Meilun Biotechnology Co., Ltd. (China). Reactive oxygen species assay kit was purchased from Beijing Solarbio Science and Technology Co., Ltd. (China). DiI, Hoechst 33342, calcein-AM/propidium iodide (PI) Kit was purchased from Suzhou Yuheng Biotechnology Co., Ltd. (China). Cell Counting (CCK8) was obtained from Boster Biological Technology Co., Ltd. (California, USA). All reagents used in this work were of analytical grade and were used without further purification.
Mitotracker green
Manufactured by Meilun
Sourced in China
MitoTracker Green is a fluorescent dye that specifically stains mitochondria in live cells. It is a lipophilic, cationic dye that accumulates in active mitochondria due to their negative membrane potential. The dye can be detected using standard fluorescein filter sets.
Automatically generated - may contain errors
Lab products found in correlation
Dspe mpeg2000, by Xi’an Ruixi (3 mentions)
Lsm 880, by Zeiss (2 mentions)
Ir780, by Merck Group (1 mentions)
1 2 dipalmitoyl sn glycerol 3 phosphocholine, by Merck Group (1 mentions)
Lysotracker green, by Meilun (1 mentions)
Cholesterol, by Merck Group (1 mentions)
Reactive oxygen species assay kit, by Solarbio (1 mentions)
Hoechst 33342, by Meilun (1 mentions)
3 protocols using mitotracker green
1
Formulation and Characterization of IR780-Loaded Liposomes
2
Intracellular Localization of TRFC NPs
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4T1 cells (1 × 106) were seeded into confocal Petri dish and incubated at 37 °C. After 24 h, the cells were co-incubated with TRFC NPs (40 μM) for different time durations ranging from 10 min to 4 h. Afterwards, the cells were washed twice with PBS to remove excess TRFC NPs, followed by incubation with MitoTracker Green (Meilunbio®, China) for 30 min. Cells were observed using confocal laser scanning microscopy (CLSM, ZEISS-LSM880) to evaluate the subcellular localization of TRFC NPs. The corresponding Pearson correlation coefficient was calculated with ImageJ software.
3
Nanorobots for Mitochondrial Targeting and Imaging
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T24 cells or 4T1 cells were seeded in cell culture dishes (2 × 105 cells per dish) and cultured overnight. The cells were cocultured with DOX, DOX-TPP, ZIF-8@DOX, ZIF-8@DOX-TPP, ZIF-67@DOX, and ZIF-67@DOX-TPP nanorobots for 12 hours. The DOX concentration in drug-loaded groups was the same as 1 μg/ml. After three-time washing with PBS, the cells were incubated with MitoTracker Green (100 nM, excitation/emission = 490/516 nm; Meilunbio) and Hoechst 33342 (1 μM) for 30 min to label mitochondria and nuclei, respectively. The fluorescent images of cells were captured using the CLSM. Five typical fluorescence images of DOX and MitoTracker Green channels were used to calculate the Pearson's correlation coefficient via ImageJ software. The nanorobots were also incubated with T24 cells for various durations (2, 4, 6, and 12 hours) to evaluate the mitochondrial targeting effect of nanorobots at different time points.
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