miR-206 mimics (miR-mimics), miR-206 inhibitor (miR-inh), and negative control (miR-NC), as well as the siRNAs against USP33 (si#1 and si#2) and control siRNA (siNC) (Supplementary Table 2 ), were obtained from GenePharma (Shanghai, China) All transfections were performed using Lipofectamine 2000 (Invitrogen) as per the manufacturer’s instruction.
Mir mimics
Manufactured by GenePharma
Sourced in China
MiR-mimics are synthetic RNA molecules designed to mimic the function of naturally occurring microRNAs (miRNAs). MiR-mimics are used as research tools to study the biological roles and regulatory effects of specific miRNAs in cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Pcdna3.1 vector, by Thermo Fisher Scientific (1 mentions)
Mir nc, by Thermo Fisher Scientific (1 mentions)
Xmark, by Bio-Rad (1 mentions)
Mir 519 mimics, by GenePharma (1 mentions)
Dimethyl sulfoxide (dmso), by Solarbio (1 mentions)
Mtt reagent, by Solarbio (1 mentions)
3 protocols using mir mimics
1
miR-206 and USP33 Modulation Protocol
2
Overexpression of GXYLT2 in Renal Cancer
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Overexpressing plasmid control oe-NC and GXYLT2 overexpressing plasmid oe-GXYLT2 (GenePharma, China) were built utilizing the pcDNA3.1 vector (Thermo Fisher Scientific, USA). Mimics NC (miR-NC) and microRNA-204-5p mimics (miR-mimics) were offered by GenePharma (China). First, 786-0 cells in logarithmic growth phase were subjected to trypsin digestion. Then, following the Lipofectamine ® 2000 (Invitrogen, USA) instructions, miR-NC and microRNA-204-5p mimics were transfected into 786-0 cells to construct the miR-NC group and miR-mimics group. Moreover, the GXYLT2 overexpression plasmid (oe-GXYLT2) and control plasmid (oe-NC) were transfected into 786-0 cells to the construct oe-GXYLT2 group and oe-NC group, respectively.
3
Investigating miR-519's Impact on Cell Viability
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To investigate the effects of miR-519 on cell viability, 5×103 cells/well were seeded in 96-well plates in 100 µl RPMI-1640 supplemented with 10% FBS, and transfected with 50 nM miR-519 mimics or 50 nM negative control miR mimics (Genepharma Co., Ltd.) for 24, 48 and 72 h, as described above. MTT reagent (20 µl; Beijing Solarbio Science & Technology Co., Ltd.) was added to the wells 24 h post-transfection, prior to a 4-h incubation in darkness. The medium was then carefully discarded and blue formazan was dissolved with 200 µl dimethyl sulfoxide (Beijing Solarbio Science & Technology Co., Ltd.), and absorbance was measured at 550 nm (xMark, Bio-Rad Laboratories, Inc., Hercules, CA, USA). Wells containing only HL60 cells served as controls.
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