Pl rink resin

Fmoc-protected amino acids were purchased from Novabiochem. PL-Rink resin was purchased from Polymer Laboratories. 1H-Benzotriazolium 1-[bis(dimethylamino) methylene]-5chloro-hexafluorophosphate (1-),3-oxide (HCTU) was obtained from Peptides International. Trifluoroacetic acid was obtained from Acros organics. 1,2-ethanedithiol was purchased from Fluka. Diethyl ether was purchased from Fisher Scientific. Tritiated thymidine [³H TdR] was purchased from Amersham Pharmacia. Cytokines were purchased from PeproTech (Rocky Hill, NJ). Heat-inactivated fetal calf serum was Hyclone purchased from Thermo. L-Glutamine and 100 U/ml penicillin and 100μg/ml streptomycin were purchased from Gibco, Tissue culture media and plastic ware was purchased from Corning Life Sciences. All other materials were purchased from Sigma/Aldrich

Fmoc-protected amino acids were purchased from Novabiochem. PL-Rink resin was purchased from Polymer Laboratories. 1H-Benzotriazolium 1-[bis(dimethylamino) methylene]-5chloro-hexafluorophosphate (1-),3-oxide (HCTU) was obtained from Peptides International. Trifluoroacetic acid was obtained from Acros organics. 1,2-ethanedithiol was purchased from Fluka. Lab-Tek™ 4-well chambered #1 borosilicate glass slides, diethyl ether, N-Methylpyrrolidone (NMP), tissue culture treated flasks and serological pipettes were purchased from Fisher Scientific. Thioanisole, anisole, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU) and 200 mM glutamine solution were obtained from Sigma-Aldrich. RPMI-1640 media and Hoechst 33342 trihydrochloride dye was purchased from Invitrogen. Heat inactivated fetal bovine serum (FBS) and trypsin EDTA were obtained from Hyclone Laboratory Inc. The parent A549 and MCF-7 cancer cell lines were obtained from the NCI-60 repository. Jurkat cells were kindly provided by Dr. Eric Freed (National Cancer Institute).

Acetic acid (ammonium salt), anisole, boric acid, deuterated water, diisopropylethylamine (DIEA), 1,2-ethanedithiol, monohydrate of 2-morpholinoethanesulfonic acid (MES), piperidine, potassium fluoride, thioanisole, and trifluoroacetic acid (TFA) were purchased from Acros. Bis-tris propane (BTP) and boric acid were purchased from Sigma-Aldrich. Sodium chloride was purchased from Fisher Scientific. O-(1H-Benzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU) and 1-hydroxybenzotriazole (HOBT) (anhydrous) were purchased from Senn Chemicals. Appropriately side-chain-protected Fmoc amino acids were purchased from Novabiochem. PL-Rink Resin was purchased from Polymer Laboratories. Sodium deuteroxide (6 M) was carefully prepared by adding small amounts of solid sodium metal to deuterated water chilled in an ice bath and then filtering away any particulate remaining in the liquid. Hydrogel formulations are represented in weight percent, as opposed to molarity, since each of the peptide’s molecular weights differ slightly. In general, a 1 wt % gel corresponds to a 2.75–3.18 mM peptide concentration range.