Trypsin edta 1 solution

Manufactured by Thermo Fisher Scientific

Sourced in Italy

Trypsin–EDTA(1×) solution is a cell culture reagent commonly used for the dissociation and detachment of adherent cells from cell culture surfaces. It contains trypsin, a proteolytic enzyme, and EDTA, a chelating agent, which work together to break down the extracellular matrix and cell-cell adhesions, enabling the release of cells from the substrate.

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Lab products found in correlation

Hepes salt, by Merck Group (1 mentions) Sephadex g 75, by Merck Group (1 mentions) Heat inactivated fbs, by Thermo Fisher Scientific (1 mentions) Tween 20 tw20, by Merck Group (1 mentions) Nh4 2so4, by Merck Group (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Mtt assay, by Promega (1 mentions) Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions) Mcf 7 and mda mb 468, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) 3 4 5 dimethylthiazol 2 yl 3 5 diphenyltetrazolium bromide mtt dye test, by Merck Group (1 mentions) Sodium dimethyl sulfoxide dmso, by Merck Group (1 mentions) Poloxamer 407 p407, by Merck Group (1 mentions)

Close spelling variants of this product

Trypsin-EDTA (7300 citations) Trypsin (6705 citations) Trypsin-EDTA solution (830 citations) Trypsin solution (210 citations) EDTA solution (66 citations) Trypsin-EDTA (1×) (47 citations) Trypsin/1 mM EDTA solution (8 citations)

3 protocols using trypsin edta 1 solution

Liposomal Doxorubicin Formulation

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Tween 20 (Tw20), Hepes salt, [N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic
acid)], polycarbonate Whatman nucleopore tracketched membranes (cut-off
0.1 μm), DPPG, Sephadex G75, (NH₄)₂SO₄, and H₂CO₃ were purchased from Sigma-Aldrich
(Sigma-Aldrich SRL, Milan, Italy). Chol and sorbitan monolaurate (Span20)
were obtained from Acros Organics products (Acros Organics BVBA Geel,
Belgium). Cellulose acetate membrane (cut-off 8 kDa) was obtained
from Prodotti Gianni S.p.A. (Milan, Italy). MTT assay was obtained
from Promega (Madison, WI, USA). DOX was obtained by D.B.A. (Milan,
Italy). MCF-7 and MDA MB 468 were obtained from American Type Culture
Collection (ATCC, LGC Standards, Teddington, UK). High-glucose Dulbecco’s
modified Eagle’s minimal essential medium (DMEM), Roswell Park
Memorial Institute Medium (RPMI-1640), heat-inactivated FBS, trypsin–EDTA
(1×) solution, and penicillin–streptomycin solution were
obtained from Gibco (Invitrogen Corporation, Giuliano Milanese, Milan,
Italy). Lissamine rhodamine B 1,2 dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (rhodamine B-DHPE)
was purchased from Invitrogen (Life Technologies Corporation, Grand
Island, NY, USA). All other chemical reagents were obtained from Sigma-Aldrich
(Milan, Italy) or Thermo Fisher Scientific (Waltham, MA USA) and are
used without further purification.

Di Francesco M., Celia C., Cristiano M.C., d’Avanzo N., Ruozi B., Mircioiu C., Cosco D., Di Marzio L, & Fresta M. (2021). Doxorubicin Hydrochloride-Loaded Nonionic Surfactant Vesicles to Treat Metastatic and Non-Metastatic Breast Cancer. ACS Omega, 6(4), 2973-2989.

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Chondrocyte Cell Culture Protocol

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Phospholipon 90G^® (PL-90G) was kindly provided by Natterman Phospholipid GMBH (Köln, Germany), and contained 93 ± 3% phosphatidylcholine. Absolute ethanol (Ph. Eur. analysis reagent) and poloxamer 407 (P407) were purchased from Sigma-Aldrich (Schnelldorf, Germany). T/C-28a2 and C-28/I2 cells, immortalized chondrocyte cell lines, were obtained from the Research Foundation for the care of cancer “Tommaso Campanella” University Campus of Germaneto–Catanzaro, I-88100, Italy. Dulbecco-modified Eagle medium (D-MEM) culture, fetal bovine serum, penicillin (100 UI/mL)–streptomycin (100 μg/mL) solution (1% v/v), and Trypsin/EDTA (1×) solution were obtained from GIBCO (Invitrogen Corporation, Giuliano Milanese, Milan, Italy). 3-[4,5-dimethylthiazol-2-yl]-3,5-diphenyltetrazolium bromide (MTT) dye test (TLC purity ≥ 97.5%), phosphate buffer (PBS) solution, and sodium dimethyl sulfoxide (DMSO), were purchased from Sigma-Aldrich (Milan, Italy).
[³H]cholesteryl hexadecyl ether ([³H]CHE, 40 Ci/mmoL) was obtained from Perkin Elmer-Italia (Monza, Italy). Double distilled pyrogen-free water was used throughout the experimental investigations. All other materials and solvents used in this study are of analytical grade (Carlo Erba, Milan, Italy).

Cristiano M.C., Mancuso A., Giuliano E., Cosco D., Paolino D, & Fresta M. (2021). EtoGel for Intra-Articular Drug Delivery: A New Challenge for Joint Diseases Treatment. Journal of Functional Biomaterials, 12(2), 34.

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Isolation and Culture of Human Chondrocytes

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Human chondrocytes were isolated from histologically healthylooking cartilage from patients undergoing total knee replacement. 18 The collection and use of human cartilage was approved by a medical ethical committee (METC) of Zorggroep Twente, The Netherlands. Human chondrocytes were cultured in T175 flasks (Cellstar®, Greiner bio-one, Germany) in chondrocyte proliferation medium (DMEM with 10% FBS (fetal bovine serum), 0.2 mM ascorbic acid 2-phosphate, 0.1 mM nonessential amino acids, 100 U ml -1 penicillin and 100 μg ml -1 streptomycin, 4 mM proline). 19, 20 Cells were passaged using a trypsin-EDTA (1×) solution (0.25%/0.1 mM, respectively, Invitrogen, Carlsbad, CA, USA) in PBS and re-seeded in a new flask at a concentration of 500 000 cells per 175 cm 2 . Cells were used at passage 4 for all experiments reported in this paper.

Paggi C.A., Hendriks J., Karperien M, & Le Gac S. (2022). Emulating the chondrocyte microenvironment using multi-directional mechanical stimulation in a cartilage-on-chip. Lab on a chip, 22(9).

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