Whole cell extracts in RIPA buffer with proteasome inhibitors were isolated for SDS-PAGE and used for the western blot analysis. Anti- p53, caspase-7, and caspase-9 antibodies were ordered from BD Pharmingen (San Diego, CA). Antibodies against total and phosphorylated H2AX, p21, and GAPDH were purchased from Abcam Inc. (Cambridge, MA). Antibodies against PARP and GSTP1 were from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-LC3 polyclonal antibody was ordered from Novus Biological Inc. (Littleton, CO).
Antibodies against total and phosphorylated h2ax
Manufactured by Abcam
Antibodies against total and phosphorylated H2AX are laboratory reagents used to detect and quantify the levels of H2AX protein and its phosphorylated form. H2AX is a histone variant that plays a critical role in the cellular response to DNA double-strand breaks. These antibodies can be used in various applications, such as Western blotting, immunohistochemistry, and flow cytometry, to investigate DNA damage and repair processes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using antibodies against total and phosphorylated h2ax
1
Investigating Cellular Stress Pathways
2
Investigating Cellular Stress Pathways
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