Elisa kit for tnf α

The following antibodies were used: anti-G6PD (Genesis Biotech, Taiwan), anti-His tag, anti-IκB, anti-IDH1, anti-β-actin, anti-HSCARG, antihorseradish peroxidase (HRP)-conjugated antimouse IgG, and antirabbit IgG (Santa Cruz Biotechnology, Santa Cruz, CA, USA). TNF-α siRNA, HSCARG siRNA, and nontargeting siRNA were purchased from Dharmacon RNA Technologies (Lafayette, CO, USA). The ELISA kit for TNF-α was purchased from R & D systems (Minneapolis, MN, USA). BAY 11-7085 and all other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Eagles’ Minimum Essential Medium (EMEM) was purchased from American Type Culture Collection (ATCC) (Manassas, VA, U.S.A.). Fetal Bovine Serum (FBS), antibiotics (penicillin and streptomycin) and trypsin were purchased from Gibco (Rockford, IL, U.S.A.). 4-Nitrophenyl N-acetyl-β-D-glucosaminide (PNAG) was purchased from Sigma Chemical Co. (St. Louis, MO, U.S.A.). The ELISA kit for TNF-α was purchased from R&D systems (Minneapolis, MN, U.S.A.). Ketotifen fumarate was purchased from Santa Cruz Biotechnology (Dallas, TX, U.S.A.). NP-40 surfact-amps detergent was purchased from Thermo Scientific (Waltham, MA, U.S.A.). Carboxymethyl cellulose (CMC) was purchased from Sigma–Aldrich (St. Louis, MO, U.S.A.). Anti-dengue IgG (pan) mouse antibody was purchased from Acris (Herford, Germany). Horse-radish peroxidase (HRP) was purchased from BioLegend (GmbH, Germany), metal enhanced 3′-diaminobenzidine (DAB) peroxidase substrate was purchased from Thermo Scientific (Pierce, Rockford, IL, U.S.A.).