HVMs, MCF-7, and HF were lysed in RIPA buffer. Total proteins (50 μg) were resolved under reducing conditions by 8–15% SDS-PAGE and transferred to Immobilon-FL membranes (Millipore). Membranes were incubated overnight at 4°C with K19 (1 : 200 dilution; Santa Cruz Biotechnology), SC-101 to detect KGFR (1 : 1000 dilution) [10 ], or with vimentin (1 : 1000 dilution; Stemgent, CA, USA) followed by goat anti-mouse or goat anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibody (Sigma-Aldrich). Bound antibody was detected by enhanced chemiluminescence detection reagents (Pierce Biotechnology Inc., Rockford, IL, USA) according to manufacturer's instructions.
Goat anti rabbit horseradish peroxidase hrp conjugated secondary antibody
Manufactured by Merck Group
Sourced in United States
Goat anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibody is a laboratory reagent used in various immunoassay techniques. It consists of a goat-derived antibody that specifically binds to rabbit primary antibodies, and is conjugated with the enzyme horseradish peroxidase. This secondary antibody can be used to detect and visualize the presence of rabbit primary antibodies in a sample.
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Lab products found in correlation
2 protocols using goat anti rabbit horseradish peroxidase hrp conjugated secondary antibody
1
Western Blot Analysis of Protein Expression
2
Serglycin Quantification in Myeloma
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Goat anti-rabbit horseradish peroxidase- (HRP-) conjugated secondary antibody was from Sigma-Aldrich. Rabbit polyclonal antibody against serglycin was prepared as previously described [7 (link)]. Chondroitinase ABC was purchased from Seikagaku. Serglycin isolated from culture medium of multiple myeloma cell lines was used as standard [7 (link)].
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