Fxiiia

Manufactured by Merck Group

Sourced in United States

FXIIIa is a laboratory equipment product from Merck Group. It is a factor that plays a key role in the blood coagulation process.

Automatically generated - may contain errors

Lab products found in correlation

Thrombin, by Merck Group (2 mentions) Tcs sp8 confocal microscope, by Leica (2 mentions) Human fibrinogen, by Merck Group (1 mentions) Urokinase type plasminogen activator u pa, by Merck Group (1 mentions) Trizma hcl, by Merck Group (1 mentions) Alexa fluor 488 conjugated fibrinogen, by Thermo Fisher Scientific (1 mentions) Trizma base, by Merck Group (1 mentions) Fd70s 100mg, by Merck Group (1 mentions)

3 protocols using fxiiia

Fibrinolysis Assay Protocol

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Human fibrinogen, thrombin, FXa, FXIIIa, urokinase-type plasminogen activator (u-PA), trizma base, and trizma HCl were purchased from Sigma-Aldrich (St. Louis, MO, USA). Paranitroaniline chromogenic substrates were obtained from Chromogenix (Milan, Italy). Alexa Fluor 488-conjugated fibrinogen was purchased from Invitrogen (Eugene, OR, USA). Other reagents used were of analytical grade and purchased from commercial sources.

Choi J.H, & Kim S. (2022). In Vitro Antithrombotic, Hematological Toxicity, and Inhibitor Studies of Protocatechuic, Isovanillic, and p-Hydroxybenzoic Acids from Maclura tricuspidata (Carr.) Bur. Molecules, 27(11), 3496.

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Fabrication of Porous MAP Scaffolds

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Fully swollen and equilibrated MAP building blocks (20 µL) were activated by with 5 U mL⁻¹ FXIIIa (Sigma) and 1 U mL⁻¹ thrombin, and the mixture was pipetted into a 3 mm diameter PDMS well on a glass coverslip and annealed in a humidified incubator at 37 °C for 1.5 h to form porous MAP scaffolds. Thereafter, the scaffolds were placed into HEPES buffer (pH 7.4) with 70 kDa dextran‐FITC (FD70S‐100MG, Sigma‐Aldrich) overnight to reach equilibrium. Samples were 3D imaged using a Leica TCS SP8 confocal microscope with 10× objective.

Miwa H., Antao O.Q., Kelly‐Scumpia K.M., Baghdasarian S., Mayer D.P., Shang L., Sanchez G.M., Archang M.M., Scumpia P.O., Weinstein J.S, & Di Carlo D. (2023). Improved Humoral Immunity and Protection against Influenza Virus Infection with a 3d Porous Biomaterial Vaccine. Advanced Science, 10(31), 2302248.

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Fabrication of Porous MAP Scaffolds

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Fully swollen and equilibrated MAP or drugMAP building blocks (20 μL) were activated by with 5 U mL⁻¹ FXIIIa (Sigma) and 1 U mL⁻¹ thrombin (Sigma), and the mixture was pipetted into a 3 mm diameter PDMS well on a glass coverslip, and annealed in a humidified incubator at 37 °C for 1.5 h to form porous MAP or drugMAP scaffolds. Thereafter, the scaffolds were placed into HEPES buffer (pH 7.4) overnight to reach equilibrium. Samples were 3D imaged using a Leica TCS SP8 confocal microscope with 10× objective, spanning 1.16 mm × 1.16 mm (in x- and y-axis) × 200 μm (in z-axis). The pore size was analyzed using a custom script written in MATLAB and the void fraction was calculated using ImageJ (stack function).

Fang J., Koh J., Fang Q., Qiu H., Archang M.M., Hasani-Sadrabadi M.M., Miwa H., Zhong X., Sievers R., Gao D.W., Lee R., Carlo D.D, & Li S. (2020). Injectable Drug-Releasing Microporous Annealed Particle Scaffolds for Treating Myocardial Infarction. Advanced functional materials, 30(43), 2004307.

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