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2 protocols using disodium ethylene diamine tetra acetic acid na2 edta

1

Synthesis and Characterization of Iron Compounds

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All of the
reagents were of analytical reagent grade, and Milli-Q water was used
throughout the experiment. Kaolin, all metal salts, iron dust, all
iron oxides, disodium ethylene diamine tetra acetic acid (Na2-EDTA), and hydrogen peroxide were purchased from Sigma-Aldrich.
Sodium borohydride was obtained from Alfa Aesar. All glassware was
cleaned with freshly prepared aqua regia, subsequently rinsed with
copious amounts of distilled water, and dried well before use. All
of the reagents were used without further purification.
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2

Phytochemical Profiling and Antioxidant Evaluation

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A Folin–Ciocalteu’s (FC) reagent, a total phenolic content (TPC) standard, iron (II) chloride hexahydrate (98%), iron (III) chloride hexahydrate (97%), iron (II) sulphate heptahydrate (99%), hydrogen chloride (HCl, 99%), sodium acetate, 2,4,6-tris-(2-pyridyl)-s-triazine (TPTZ > 98%), sinapic acid (>97%), 2,2-diphenyl-1-picrylhydrazyl (DPPH, 97%), and formic acid were all purchased from Fisher Scientific Canada Ltd. (Ottawa, ON, Canada). Aluminum chloride (AlCl3), sodium nitrite (NaNO2), sodium carbonate (Na2CO3), sodium hydroxide (NaOH), ferrozine, and disodium ethylenediaminetetraacetic acid (Na2EDTA) were purchased from Sigma Canada Ltd. (Mississauga, ON, Canada). Quercetin hydrate (>95%) and 2-amino-ethyl-diphenyl borate (98%) were purchased from Acros (Mississauga, ON, Canada).
Extraction reagents, including methanol (optima grade) and ethanol (analytical grade), and standard compounds for high-performance liquid chromatography (HPLC) were purchased from Sigma Canada Ltd. (Mississauga, ON, Canada).
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