Edta coated

Manufactured by Greiner

Sourced in Austria

EDTA-coated lab equipment is designed to prevent sample clotting. EDTA, or Ethylenediaminetetraacetic acid, is a chelating agent that binds to calcium ions, inhibiting the coagulation cascade. This product is suitable for various applications that require uncoagulated samples, such as blood analysis and cell culture studies.

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Lab products found in correlation

Minicollect blood collection tubes, by Greiner (1 mentions) Hemavet 950fs, by Drew Scientific (1 mentions)

Spelling variants of this product

EDTA-coated tubes (7 citations) K3-EDTA-coated tubes (3 citations) EDTA‐coated vacutainer tubes (2 citations)

2 protocols using edta coated

Complete Blood Count Analysis in Mice

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The generation of Stk4 KO mice and the flow cytometry experiments follow the protocols that we have described elsewhere [11 (link)]. For the Complete Blood Count (CBC) analysis, blood was collected into EDTA-coated MiniCollect blood collection tubes (Greiner Bio-One, Kremsmünster, Austria, catalog #VG-450474) via submandibular vein puncture from unanesthetized mice. 40 μL of blood from each mouse were aliquoted and analyzed by a Hemavet 950FS (Drew Scientific, Oxford, CT, USA). Using an electrical impedance and focused flow cell system, the Hemavet quantified the total number of white blood cells, eosinophils, neutrophils, basophils, and lymphocytes.

Zhang Z., Luo D., Zhong X., Choi J.H., Ma Y., Wang S., Mahrt E., Guo W., Stawiski E.W., Modrusan Z., Seshagiri S., Kapur P., Hon G.C., Brugarolas J, & Wang T. (2019). SCINA: A Semi-Supervised Subtyping Algorithm of Single Cells and Bulk Samples. Genes, 10(7), 531.

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Evaluating Metabolic Changes in Mice

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Mice of different experimental groups were weighed daily until the end of the first week and every two days until the end of the experiment. The body weight change was calculated and recorded as mean ± standard deviation (SD). Blood was collected in EDTA coated K2 tubes (Greiner Bio-one, Monroe, NC) from all groups at the end of the study and different parameters were measured. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were assessed as indicator of liver damage and serum creatinine and blood urea nitrogen as indicators of kidney function. WBC and platelets were also counted. All these parameters were measured using the kits mentioned in the materials section and used accordingly to the manufacturer’s instructions.

Nascimento A.V., Singh A., Bousbaa H., Ferreira D., Sarmento B, & Amiji M.M. (2016). Overcoming Cisplatin Resistance in Non-Small Cell Lung Cancer with Mad2 Silencing siRNA Delivered Systemically using EGFR-Targeted Chitosan Nanoparticles. Acta biomaterialia, 47, 71-80.

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