Whole blood and isolated colonic LPL cells from CCR2-GFP mice were stained with PE-Conjugated Ly6C (Clone-AL/21) and APC-Conjugated CD11b+ (Clone-M1/70) (BD Pharmingen, San Jose, CA) at 4°C for 45 minutes. FACS Lysing Solution (BD, San Jose, CA) was added to the cells after incubation for 10 minutes, then a single rinse/spin cycle (400g for 5 min) was performed with FACS Buffer. Samples were analyzed with the BD FACSCalibur dual-laser flow cytometer (BD Immunocytometry Systems, San Jose, CA) with CellQuest Pro. Cells were gated based on forward and side-scatter and CD11b expression and classified based on Ly6C and GFP-CCR2 expression levels.
Pe conjugated ly6c clone al 21
Manufactured by BD
PE-Conjugated Ly6C (Clone-AL/21) is a laboratory instrument designed for the detection and quantification of Ly6C, a cell surface marker, in biological samples. This product is conjugated with the fluorescent dye Phycoerythrin (PE) to enable visualization and analysis using flow cytometry or other fluorescence-based techniques.
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2 protocols using pe conjugated ly6c clone al 21
1
Colonic Immune Cell Profiling
2
Colonic Immune Cell Profiling
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Whole blood and isolated colonic LPL cells from CCR2-GFP mice were stained with PE-Conjugated Ly6C (Clone-AL/21) and APC-Conjugated CD11b+ (Clone-M1/70) (BD Pharmingen, San Jose, CA) at 4°C for 45 minutes. FACS Lysing Solution (BD, San Jose, CA) was added to the cells after incubation for 10 minutes, then a single rinse/spin cycle (400g for 5 min) was performed with FACS Buffer. Samples were analyzed with the BD FACSCalibur dual-laser flow cytometer (BD Immunocytometry Systems, San Jose, CA) with CellQuest Pro. Cells were gated based on forward and side-scatter and CD11b expression and classified based on Ly6C and GFP-CCR2 expression levels.
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