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1260 infinity quaternary lc apparatus

Manufactured by Agilent Technologies

The 1260 Infinity Quaternary LC apparatus is a high-performance liquid chromatography (HPLC) system designed for analytical and preparative applications. It features a quaternary solvent delivery system that allows for the precise and accurate delivery of up to four different solvents, enabling advanced gradient capabilities. The system is capable of operating at a maximum pressure of 600 bar and a flow rate range of 0.001 to 10 mL/min, making it suitable for a wide variety of chromatographic separations.

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2 protocols using 1260 infinity quaternary lc apparatus

1

Analytical Characterization of Organic Compounds

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Optical rotations were measured using a Jasco P-2000 polarimeter at the sodium D line. ECD spectra were recorded using a Jasco-715 (Jasco Europe, Carpi, Italy) spectropolarimeter. NMR spectra were determined on Varian Unity Inova (Varian ,Palo Alto, CA, USA)pectrometers at 700 MHz and 500 MHz; chemical shifts were referenced to the residual solvent signal (CD3OD: δH 3.31, δC 49.30). For an accurate measurement of the coupling constants, the one-dimensional 1H-NMR spectra were transformed at 64-K points (digital resolution: 0.09 Hz). The HSQC spectra were optimized for 1JCH = 142 Hz; the 13C and 31P HMBC experiments for 2,3JCH = 8.3 Hz and 3JPH = 8 Hz, respectively. High-resolution ESI-MS and HR-ESI-HPLC experiments were performed on a Thermo LTQ Orbitrap XL mass spectrometer coupled to a Thermo U3000 HPLC system. Fatty acid methyl esters (FAMEs) were analyzed by GC/MS (Agilent, Cernusco sul Naviglio (Mi), Italy, 6850 series II/5973 Network MSD) on an HP-5MS capillary column (Agilent, 5% Phenyl Methyl Siloxane) (30 m, 0.25 mm φ, 0.25 μm). Helium was used as a carrier gas, injection was in split mode, and the program was as follows: hold 150 °C for 15 min, heat to 300 °C with 5 °C/min, hold 300 °C for 10 min. High performance liquid chromatography (HPLC) separations were achieved on an Agilent 1260 Infinity Quaternary LC apparatus equipped with a Diode-Array Detector (DAD).
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2

Characterization of Conulothiazoles via NMR

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Optical rotations were measured using a Jasco P-200 polarimeter. UV spectra were measured using a Beckman Coulter DU-800 spectrophotometer. ECD spectra were recorded on a Jasco J-715 spectrophotometer using a 1-mm cell. NMR spectra were determined on Varian Unity Inova spectrometers at 700 MHz; chemical shifts were referenced to the residual solvent signal (CD3OD: δH 3.31, δC 49.00). Other NMR data were recorded using a Bruker 800 MHz NMR and a Varian 500 MHz NMR; chemical shifts were referenced to the residual solvent signal (DMSO-d6: δH 2.51, δC 39.5). For an accurate measurement of the coupling constants, the one-dimensional 1H NMR spectra were transformed at 64 K points (digital resolution: 0.09 Hz). Through-space 1H connectivities were evidenced using a ROESY or NOESY experiment with a mixing time of 450 ms. The HSQC spectra were optimized for 1JCH = 142 Hz, and the HMBC experiments for 2 (link),3 JCH = 8.3 Hz. HRESIMS analysis of the new conulothiazoles was performed using an AB SCIEX TripleTOF 4600 mass spectrometer with Analyst TF software. High performance liquid chromatography (HPLC) separations were achieved on an Agilent 1260 Infinity Quaternary LC apparatus equipped with a Diode-Array Detector (DAD). The new conulothiazoles were isolated using a Dionex UltiMate 3000 HPLC system each equipped with a micro vacuum degasser, an autosampler, and a DAD.
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