Log In Sign up
The largest database of trusted experimental protocols

Cacna1c sirna

Manufactured by Santa Cruz Biotechnology
Visit Supplier

CACNA1C siRNA is a small interfering RNA (siRNA) that targets the CACNA1C gene, which encodes the alpha-1C subunit of the L-type voltage-gated calcium channel. This siRNA can be used to experimentally downregulate the expression of the CACNA1C gene.

Automatically generated - may contain errors

Lab products found in correlation

Control sirna, by Santa Cruz Biotechnology (1 mentions) Opti mem, by Thermo Fisher Scientific (1 mentions) Rnaimax, by Thermo Fisher Scientific (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

SiRNAs (95 citations) CACNA1C (3 citations)

2 protocols using cacna1c sirna

1

Silencing CACNA1C in AM Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
AM cells were prepared on 0.1% gelatin-coated 6 well plates at a density of 200,000 cells/well in KGM-2. CACNA1C siRNA (sc-42688, Santa Cruz) or Control siRNA (scrambled, sc-37007 Santa Cruz) was added to Opti-MEM (Gibco) at 20 nM concentration. RNAiMAX (Invitrogen) was also added to the Opti-MEM by 0.125 µL per unit area (cm2) of culture dish according to the manufacturer’s instructions. AM cells were incubated with siRNA containing Opti-MEM for 6 h. The cells were gently washed with DPBS then the medium was changed to fresh KGM®-2. Cells were harvested for further analyses at 72 h after knockdown.
Li S., Lee D.J., Kim H.Y., Kim J.Y., Jung Y.S, & Jung H.S. (2022). Unraveled roles of Cav1.2 in proliferation and stemness of ameloblastoma. Cell & Bioscience, 12, 145.
+ Open protocol
+ Expand
2

Silencing CACNA1C in AM Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The AM cells were transfected with either negative control siRNA (Scrambled; sc‐398433; Santa Cruz) or CACNA1C siRNA (sc‐42688; Santa Cruz) following the manufacturer's protocol for lipofectamine RNAiMAX (Life Technologies). For the siRNA experiments, 2 × 105 cells were seeded in each six‐well culture dish. Once cells had grown to 80% confluence, a mixture of 4 μl of siRNA (40 pmol/μl) and 6 μl of Lipofectamine 2000 reagent (Invitrogen) was added to the medium of each dish. After 48 h, cells were harvested for the ICC, spheroid invasion and migration assays.
Li S., Kim H., Lee D., Park S., Otsu K., Harada H., Jung Y, & Jung H. (2022). Inhibition of L‐type voltage‐gated calcium channel‐mediated Ca2+ influx suppresses the collective migration and invasion of ameloblastoma. Cell Proliferation, 55(11), e13305.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!