All chemicals (amines, alkyl thiols, and solvents) for the synthesis of functional polyesters were purchased from Sigma-Aldrich (Burlington, MA, USA), TCI America (Portland, OR, USA), or Fisher Scientific (Hampton, NH, USA). Luciferase and Cre mRNA were obtained from TriLink Biotechnologies (San Diego, CA, USA). RPMI-1640, fetal bovine serum (FBS), phosphate buffered saline (PBS), and PEO101−PPO56−PEO101 (Pluronic F-127, Mw = 12 600, PDI = 1.05) were purchased from Sigma-Aldrich. DMG-PEG lipid (Sunbright GM-020) was purchased from NOF America (White Plains, NY, USA). The Quant-iT RiboGreen RNA assay kit was obtained from Life Technologies (Carlsbad, CA, USA). The ONE-Glo + Tox luciferase assay kit was obtained from Promega (Madison, WI, USA).
Peo101 ppo56 peo101 pluronic f 127
Manufactured by Merck Group
PEO101−PPO56−PEO101 (Pluronic F-127) is a triblock copolymer consisting of a central hydrophobic polypropylene oxide (PPO) block flanked by two hydrophilic polyethylene oxide (PEO) blocks. It is a non-ionic surfactant with a critical micelle concentration (CMC) and cloud point that varies with concentration and temperature.
Automatically generated - may contain errors
Lab products found in correlation
One glo tox luciferase assay kit, by Promega (2 mentions)
Quant it ribogreen rna assay kit, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Merck Group (1 mentions)
Organic solvents, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Matrigel, by BD (1 mentions)
2 protocols using peo101 ppo56 peo101 pluronic f 127
1
Synthesis and Characterization of Functional Polyesters
2
Synthesis and Characterization of Polymeric Nanoparticles
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Organic solvents were purchased from Fisher Scientific or Sigma-Aldrich and used as received. Amines, thiols, trimethylolpropane allyl ether (TPAE), suberoyl chloride (diACl-C 8 ), 2,2-dimethoxy-2-phenylacetophonone (DMPA), γ-thiobutyrolactone (TBL) Dulbecco's Modified Eagle Medium (DMEM), fetal bovine serum (FBS), and phosphate buffered saline (PBS) were purchased from Sigma-Aldrich. 4',6-Diamidino-2-phenylindole (DAPI), LysoTracker Green, and Quant-iT RiboGreen RNA assay kits were purchased from Life Technologies. The ONE-Glo + Tox luciferase assay kit was purchased from Promega. Matrigel was purchased from BD Biosciences. Custom-synthesized siRNA against Luciferase (siLuc) (sense: 5'-GAUUAUGUCCGGUUAUGU A[dT][dT]-3'; antisense: 5'-UACAUAACCGGACAUAAUC [dT][dT]-3'), and Cy5.5-labeled siRNA (Cy5.5-siRNA) (sense: 5'-GAUUAUGUCCGGUUAUGUA[dT][dT]-Cy5.5-3'; antisense: 5'-UACAUAACCGGACAUAAUC[dT][dT]-3') were purchased from Sigma-Aldrich. PEO 80 -PPO 27 -PEO 80 (Pluronic F-68, M w = 8,400, PDI = 1.06) and PEO 101 -PPO 56 -PEO 101 (Pluronic F-127, M w = 12,600, PDI = 1.05) were obtained from Sigma-Aldrich. PEG 2000 DMG lipid (Sunbright GM-020, 1,2-dimyristoyl-snglycerol, methoxypolyethylene glycol) was purchased from NOF America.
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