D β hydroxybutyrate

Manufactured by Merck Group

Sourced in United States

D-β-hydroxybutyrate is a biochemical compound that can be used in various laboratory applications. It is a ketone body, which are produced during the breakdown of fatty acids and can serve as an alternative energy source for certain cells. This product is suitable for use in assays, experiments, and other research-related activities where the measurement or analysis of D-β-hydroxybutyrate is required.

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Lab products found in correlation

Acetoacetate, by Merck Group (1 mentions) Acquity hss t3 column, by Waters Corporation (1 mentions) Empower3 software suite, by Waters Corporation (1 mentions) Qda mass detector, by Waters Corporation (1 mentions) Acquity uplc h class system, by Waters Corporation (1 mentions) Chloroquine, by Merck Group (1 mentions) β nad, by Merck Group (1 mentions)

2 protocols using d β hydroxybutyrate

Quantitative Analysis of TCA Cycle Intermediates

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TCA cycle intermediates were quantified in MCEC treated with 2 mM acetoacetate or 2 mM D‐β‐hydroxybutyrate (Sigma‐Aldrich) or the respective controls for 24 h. Samples were extracted in methanol with sonication and the separation protocol was adapted from Uran et al (2007 (link)). In brief, samples were analyzed on an Acquity HSS T3 column (Waters) heated to 40°C connected to an Acquity H‐class UPLC system (Waters). The UPLC system was coupled to a QDa mass detector (Waters) in single ion record mode, using negative detector polarity and 0.8 kV capillary voltage. Data acquisition and processing was performed with the Empower3 software suite (Waters).

Weis E., Puchalska P., Nelson A.B., Taylor J., Moll I., Hasan S.S., Dewenter M., Hagenmüller M., Fleming T., Poschet G., Hotz‐Wagenblatt A., Backs J., Crawford P.A, & Fischer A. (2022). Ketone body oxidation increases cardiac endothelial cell proliferation. EMBO Molecular Medicine, 14(4), e14753.

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Neuronal Metabolism and Signaling Pathways

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After 8 DIV neurons were incubated with the following compounds: 6 to 48 h with D-β-hydroxybutyrate (5 mM; Sigma-Aldrich, 298360-1G), 24 h with L-β-hydroxybutyrate (5 mM; Sigma-Aldrich, H3145-1G), 24 h with β-NAD (5 mM; Sigma-Aldrich, N-7004), 4 h with chloroquine (20 µM; Sigma-Aldrich, C6628-25G) and 24 h with AGK2 (SIRT2 inhibitor, 5 µM; Merk Millipore, 566324-5MG, Burlington, MA, USA).

Gómora-García J.C., Montiel T., Hüttenrauch M., Salcido-Gómez A., García-Velázquez L., Ramiro-Cortés Y., Gomora J.C., Castro-Obregón S, & Massieu L. (2023). Effect of the Ketone Body, D-β-Hydroxybutyrate, on Sirtuin2-Mediated Regulation of Mitochondrial Quality Control and the Autophagy–Lysosomal Pathway. Cells, 12(3), 486.

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