The eLUCidate HRE reporter cell line, a stably transfected HeLa cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the hypoxia response element (HRE), was obtained from Genlantis (San Diego, CA). The cells were seeded in a 96-well plate at 1 × 104 cells per well and treated with various exosomes at 50 μg/ml for 48 h. The HRE signals were detected by using a Renilla Luciferase Glow Assay Kit (Thermo Fisher Scientific). Briefly, the cells were rinsed with PBS once. Cell lysis buffer was added at 50 μl/well followed by shaking on a shaker for 15 min. For each well, 20 μl of the cell lysate was transferred to an opaque 96-well plate and incubated with 50 μl of working solution for 10 min. The light output was detected with a SpectraMax M5 microplate reader (Molecular Devices, San Jose, CA).
Elucidate hre reporter cell line
Manufactured by Genlantis
The ELUCidate HRE reporter cell line is a tool for studying hypoxia response elements (HREs) and hypoxia-inducible factor (HIF) signaling. The cell line contains a reporter gene that is under the control of an HRE promoter, allowing for the measurement of HIF transcriptional activity.
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Lab products found in correlation
2 protocols using elucidate hre reporter cell line
1
Hypoxia Response Element Assay
2
Hypoxia Reporter Assay with Exosomes
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The eLUCidate HRE reporter cell line, a stably transfected HeLa cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the hypoxia response element (HRE), was obtained from Genlantis (San Diego, CA). The cells were seeded in a 96well plate at 1x10 4 cells per well and treated with various exosomes at 50 μg/ml for 48 hours. The HRE signals were detected by using a Renilla Luciferase Glow Assay Kit (Thermo Fisher Scientific). Briefly, the cells were rinsed with PBS once. Cell lysis buffer was added at 50 μl/well followed by shaking on a shaker for 15 min. For each well, 20 μl of the cell lysate was transferred to an opaque 96-well plate and incubated with 50 μl of working solution for 10 min. The light output was detected with a SpectraMax M5 microplate reader (Molecular Devices, San Jose, CA).
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