Anti c fos primary antibody

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The Anti-c-Fos primary antibody is a research-use tool designed to detect the presence and expression levels of the c-Fos protein in biological samples. c-Fos is an immediate early gene that serves as a marker of neuronal activation. This antibody can be used in various techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to study the expression and localization of the c-Fos protein.

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Lab products found in correlation

Lsm 510, by Zeiss (2 mentions) Fluorescein isothiocyanate (fitc), by Abcam (1 mentions) Goat anti mouse secondary antibody conjugated to cy3, by Jackson ImmunoResearch (1 mentions) Normal goat serum (ngs), by Vector Laboratories (1 mentions) Biotinylated goat anti rabbit igg, by Vector Laboratories (1 mentions) Dapi dye, by Thermo Fisher Scientific (1 mentions) Sc 52, by Santa Cruz Biotechnology (1 mentions) Prolong gold antifade reagent, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

C-Fos (129 citations) Anti-c-Fos (68 citations) C-Fos antibody (14 citations) Anti-Fos (7 citations) C-fos primary antibody (6 citations)

4 protocols using anti c fos primary antibody

Orexin-A Activation Induces c-Fos in NPY Neurons

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Rats were subjected to unilateral ARC orexin-A (n = 8; 0.5 μg) or vehicle injections (n = 5). Ninety minutes later, rats were euthanized and their brains were removed and processed, according to the procedures outlined in Immunohistochemistry.
C-Fos is regarded as a marker of neuronal activity, and immunohistochemical detection was performed following the procedure described in Immunohistochemistry, using a mouse monoclonal anti-c-Fos primary antibody (1:100, Santa Cruz Biotechnology, CA, United States, cat# sc-8047), followed by a goat anti-mouse secondary antibody conjugated to Cy3 (1:500, Jackson ImmunoResearch, PA, United States, cat# 115-165-003). Immunohistochemistry detection of NPY was performed using a rabbit anti-NPY polyclonal antibody (1:500, Abcam, Cambridge, United Kingdom, cat# ab30914), and was followed by incubation of a goat anti-rabbit secondary antibody (FITC, 1:1000, Abcam, Cambridge, United Kingdom, cat# ab6717).
Quantification of colocalization of c-Fos and NPY positive cells was processed according to the means described above. This calculation was based on the number of co-localized c-Fos and NPY cells divided by the total number of NPY positive cells.

Guo F., Gao S., Xu L., Sun X., Zhang N., Gong Y, & Luan X. (2018). Arcuate Nucleus Orexin-A Signaling Alleviates Cisplatin-Induced Nausea and Vomiting Through the Paraventricular Nucleus of the Hypothalamus in Rats. Frontiers in Physiology, 9, 1811.

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Mapping Psychostimulant-Induced Neural Activity

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Psychostimulant-induced c-Fos immunohistochemistry was performed in order to determine whether RGS12-null mice showed a reduction in neural activity specifically within the NAc of the vSTR. Mice received ip injection of saline or d-AMPH (3 mg/kg). Following 90 min, mice were anesthetized with pentobarbital (200 mg/kg) and transcardially perfused with 4% paraformaldehyde. Perfused brains were post-fixed overnight and then placed in 30% sucrose solution until brains sank. Cryoprotected brains were then frozen overnight at −80°C and 40-μm free-floating sections were prepared. Sections were incubated with a polyclonal anti-c-Fos primary antibody (1:100; Santa Cruz, Dallas, Texas, USA) overnight at 4°C, followed by incubation of a flurophore-conjugated goat anti-rabbit secondary antibody (Thermo Fisher Scientific, Waltham, MA, USA) for two hours at RT. Psychostimulant-induced c-Fos expression was visualized on an Evos epifluorescent microscope and c-Fos-positive nuclei were counted in a 0.16-mm² field of the dSTR and vSTR. Anatomical landmarks, such as the anterior commissure, corpus callosum, and septum, were utilized to demarcate the dSTR and vSTR. Three independent sections separated by 200 μm were counted per mouse, and presented data were determined by computing the average number of c-Fos-positive nuclei per field in 3–4 mice per genotype.

Gross J.D., Kaski S.W., Schroer A.B., Wix K.A., Siderovski D.P, & Setola V. (2018). Regulator of G protein signaling-12 modulates the dopamine transporter in ventral striatum and locomotor responses to psychostimulants. Journal of psychopharmacology (Oxford, England), 32(2), 191-203.

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Immunohistochemical Analysis of c-Fos Expression

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Ten rats were used, 5 naïve and 5 submitted to salicylate treatment. Rats were perfused transcardially with 0.9% NaCl followed by 4% paraformaldehyde in 0.1 M phosphate buffer (pH 7.4). The brain was removed, postfixed in the same solution for 1 h, and cryoprotected with 30% sucrose in 0.1 M phosphate buffer for 2 days at 4 °C. The brains were individually sectioned (50 μm) in the transverse plane using a cryostat.
The sections were washed in PBS (0.01 M, pH 7.4) and incubated for 30 min in PBS containing 1% hydrogen peroxide to inactivate endogenous peroxidases. After several rinses in PBS for 30 min, the sections were placed in 5% normal goat serum (Vector) for 1 h and incubated for 48 h at 4 °C with primary anti c-fos antibody generated in rabbits (1:100; Santa Cruz). After rinsed in PBS, the sections were incubated for 1.5 h at room temperature with biotinylated goat anti-rabbit IgG (1:150; Vector); then, subsequently, washed in PBS and placed for 1.5 h in avidin–biotin peroxidase complex (Vectastain, Vector). The immunolabeling was revealed by 5–10 min of incubation with 0.05% 3,3′-diaminobenzidine tetrachloride and 0.1% hydrogen peroxide. The sections were mounted on gelatin-coated slides, dehydrated, cleared with xylene and coverslipped with Entellan^®.

Santos P., da Silva L.E, & Leão R.M. (2016). Specific immediate early gene expression induced by high doses of salicylate in the cochlear nucleus and inferior colliculus of the rat. Brazilian Journal of Otorhinolaryngology, 83(2), 155-161.

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Immunofluorescence Labeling of c-Fos in Mouse Brain

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Mice were deeply anesthetized with ketamine/xylazine and sacrificed, then transcardially perfused with cold phosphate-buffered saline (PBS) followed by cold 4% paraformaldehyde (PFA) in 1 x PBS. The brains were dissected and post-fixed in 4% PFA in PBS at 4°C overnight. Free-floating vibratome coronal sections (35 μm) were cut and incubated in a blocking solution containing 10% normal donkey serum, 0.2% Triton X-100, 3% bovine serum albumin, and 0.02% sodium azide in 1 x PBS for 2 hr at room temperature (RT). Sections were labeled with primary anti-c-Fos antibody (1:400; sc-52; Santa Cruz Biotechnology, USA) in the blocking solution at 4°C overnight, followed by the Alexa488-conjugated (1:1000; Invitrogen, ThermoFisher Scientific) secondary antibody for 1 hr at RT. Slices were incubated for 20min in 1 x DAPI dye (Invitrogen, ThermoFisher Scientific) in PBS at RT to label cell nuclei. Samples were then washed 4 × 15 min in PBS with 0.1% Triton X-100. Immunolabeled brain sections were mounted onto glass slides using ProLong Gold anti-fade reagent (Invitrogen, ThermoFisher Scientific, USA) and stored at −20°C. Images of the cortical and hippocampal areas from the stimulated and unstimulated sides of the brain were acquired using a high-resolution multi-channel (sequential) scanning confocal microscope (LSM 510, Zeiss, Germany), using a 10x air objective (NA 0.45).

Grossman N., Bono D., Dedic N., Kodandaramaiah S.B., Rudenko A., Suk H.J., Cassara A.M., Neufeld E., Kuster N., Tsai L.H., Pascual-Leone A, & Boyden E.S. (2017). Noninvasive Deep Brain Stimulation via Temporally Interfering Electric Fields. Cell, 169(6), 1029-1041.e16.

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