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Libs 2

Manufactured by Merck Group

LIBS-2 is a laboratory equipment product designed for spectroscopic analysis. It utilizes Laser-Induced Breakdown Spectroscopy (LIBS) technology to determine the elemental composition of samples. The core function of LIBS-2 is to provide accurate and rapid analysis of materials without the need for sample preparation.

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2 protocols using libs 2

1

Biotinylated Fibronectin Binding Assay

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Biotinylated FNIII7-10 was a gift from Dr. Andres Garcia (Georgia Tech, GA). Human plasma fibrinogen was a gift from Dr. Shaun Jackson (The University of Sydney, Australia). The antibody AP3 was a generous gift from Dr. Peter Newman (Blood Center of Wisconsin, WI). The antibody LIBS-2 was purchased from EMD Millipore (Billerica, MA). The anti-mouse β1 blocking Ab HMβ1-1 was purchased from Biolegend (San Diego, CA).
MAL-PEG3500-NHS and Biotin-PEG3500-NHS were purchased from JenKem (Plano, TX). Nystatin, streptavidin–maleimide and BSA were purchased from Sigma-Aldrich (St. Louis, MO). Borosilicate glass beads were purchased from Distrilab Particle Technology (RC Leusden, The Netherlands).
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2

Integrin-Mediated Cell Adhesion Assay

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Previously described16 (link),32 (link) recombinant α5β1-Fc and trα5β1-Fc were generous gifts of Martin J. Humphries
(University of Manchester, UK),54 (link) αVβ3-Hexa-His was a kind gift of Junichi Takagi
(Osaka University, Japan).28 (link) α5β1-Poly-His was purchased from Sino Biological
(Wayne, PA). FN biotinylated at the N-terminus was a kind gift of
Andres J. Garcia (Georgia Institute of Technology, USA).55 (link) The anti-FN mAb (HFN7.1) was from Developmental
Studies Hybridoma Bank (Iowa City, IA). The antihuman Fc capturing
mAb (GG-7) was from Sigma-Aldrich (St. Louis, MO). LIBS-2 and BMC5
was purchased from EMD Millipore (Billerica, MA). Anti-Penta-His antibody
was purchased from Qiagen (Germany).
MAL-PEG3500-NHS and Biotin-PEG3500-NHS
were from JenKem (Plano, TX). Nystatin, streptavidin-maleimide, and
BSA were from Sigma-Aldrich. Borosilicate glass beads were from Distrilab
Particle Technology (RC Leusden, The Netherlands).
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