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Peqlab gold total rna kit

Manufactured by Avantor
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Sourced in Germany

The Peqlab-Gold Total RNA-Kit is a laboratory equipment product designed for the extraction and purification of total RNA from various biological samples. It provides a reliable and efficient method for obtaining high-quality RNA, which is an essential step in various molecular biology and genomic research applications.

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Lab products found in correlation

Roti quick kit, by Carl Roth (1 mentions) Sybr green lc480 system, by Roche (1 mentions) Platinum sybr green qpcr supermix, by Thermo Fisher Scientific (1 mentions) Stepone plus 96 well rt pcr system, by Thermo Fisher Scientific (1 mentions) Mulv reverse transcriptase, by Thermo Fisher Scientific (1 mentions)

2 protocols using peqlab gold total rna kit

1

RNA Extraction and Quantitative RT-PCR

Cited 1 time
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RNA from cells or tissue powder was isolated using the Roti®-Quick-Kit (Carl Roth). RNA extracted from tissue powder was purified using the Peqlab-Gold Total RNA-Kit (12-6834-01, Peqlab). Reverse transcription and quantitative RT-PCR using the SYBR Green LC480 system (Roche) was conducted as previously described41 (link). Primers are listed in Supplementary Table 2 and RPL19 or HPRT served as housekeeping genes for relative gene expression analysis.
Welk V., Meul T., Lukas C., Kammerl I.E., Mulay S.R., Schamberger A.C., Semren N., Fernandez I.E., Anders H.J., Günther A., Behr J., Eickelberg O., Korfei M, & Meiners S. (2019). Proteasome activator PA200 regulates myofibroblast differentiation. Scientific Reports, 9, 15224.
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2

Gene Expression Analysis of Lung Tissue

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Total RNA from lung tissue homogenate was isolated using the peqlab-Gold Total RNA-Kit (Peqlab, Erlangen, Germany, #12-6834-01) before cDNA synthesis (random hexamers, MuLV reverse transcriptase (Applied Biosystems, Germany)). mRNA expression of target and housekeeping gene (hypoxanthine-guanine phosphoribosyltransferase, HPRT-1) expression was determined by Platinum SYBR Green qPCR SuperMix (Applied Biosystems; StepOnePlus 96 well RTPCR System (Applied Biosystems, CA) and displayed as 2−ΔCT (ΔCT = CT target–CT reference) or as 2−ΔΔCT values (ΔΔCT = ΔCT treated–ΔCT control).
Heydarian M., Oak P., Zhang X., Kamgari N., Kindt A., Koschlig M., Pritzke T., Gonzalez-Rodriguez E., Förster K., Morty R.E., Häfner F., Hübener C., Flemmer A.W., Yildirim A.O., Sudheendra D., Tian X., Petrera A., Kirsten H., Ahnert P., Morrell N., Desai T.J., Sucre J., Spiekerkoetter E, & Hilgendorff A. (2022). Relationship between impaired BMP signalling and clinical risk factors at early-stage vascular injury in the preterm infant. Thorax, 77(12), 1176-1186.
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