Hydrogen tetrachloroaurate (III) trihydrate (HAuCl4·3H2O, 99.9%), trisodium citrate (Na3C6H5O7·2H2O), and anti-BSA antibodies produced in rabbits were purchased from Sigma-Aldrich (St. Louis, MO, USA). BSA was purchased from HIMEDIA (Mumbai, India). Phosphate buffer saline (PBS) without Ca2+ and Mg2+ was made by NacalaiTesque (Kyoto, Japan). Borate buffer (pH 7.4), boric acid (H3BO3), polyoxyethylene (20) sorbitan monolaurate, and sodium azide were purchased from OmniPur (Darmstadt, Germany). Trizma® HCl·C4H11NO3; 99% and sucrose were purchased from MERK (Darmstadt, Germany). Millipore CO48 and backing cards were purchased from MERK (Darmstadt, Germany). STANDARD 14 and AE-99 were purchased from GE Healthcare (Buckinghamshire, UK). Supporter sheets and AE-99 were kindly supported by Bang Trading 1992 Co., Ltd. (Bangkok, Thailand).
Anti bsa antibody
Manufactured by Merck Group
Sourced in United States
Anti-BSA antibodies are laboratory reagents used to detect and quantify bovine serum albumin (BSA) in various biological samples. They can be used in various immunoassay techniques, such as ELISA, Western blotting, and immunohistochemistry, to measure the presence and concentration of BSA.
Automatically generated - may contain errors
Lab products found in correlation
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Trisodium citrate, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by HiMedia (1 mentions)
Hydrogen tetrachloroaurate 3 trihydrate, by Merck Group (1 mentions)
Phosphate buffer saline (pbs), by Nacalai Tesque (1 mentions)
Fv1000, by Olympus (1 mentions)
Sytox green, by Thermo Fisher Scientific (1 mentions)
Hank s balanced salt solution hbss, by Thermo Fisher Scientific (1 mentions)
Wortmannin, by Merck Group (1 mentions)
Staurosporine, by Merck Group (1 mentions)
Lipopolysaccharide lps from e coli 055 b5, by Merck Group (1 mentions)
Misoprostol, by Cayman Chemical (1 mentions)
Dibutyryl cyclic amp dbcamp, by Merck Group (1 mentions)
Equine recombinant granulocyte monocyte colony stimulating factor gm csf, by Kingfisher Biotech (1 mentions)
4 protocols using anti bsa antibody
1
Fabrication of Gold Nanoparticles for Biosensing
2
Quantifying NET-like Structures in Mouse Cremaster
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Analysis of NET-like structures was performed as described previously (Chen et al., 2012 (link)). Briefly, male eight-week-old WT C57BL6/j mice were treated with 20 mg/kg bovine Lf or PBS alone, followed by administration of an intrascrotal injection of anti-BSA antibodies (200 μg/300 μL, Sigma-Aldrich) and an intravenous injection of BSA (300 μg/100 μL, Sigma-Aldrich) to induce RPA reaction as previously reported (Stokol et al., 2004 (link)). After 3 h, mice were injected with SYTOX Green (25 nM, Molecular Probes, USA), and the cremaster muscle was prepared for intravital microscopy. Images were obtained using an upright epifluorescence microscope (FV1000, Olympus Imaging, USA) with a 40 × water-immersion objective. Images were recorded with a CCD camera (DP72, Olympus Imaging) and analyzed using ImageJ software (NIH). SYTOX Green-positive individual NET fibers were counted in 8–10 representative images (436 μm × 328 μm). Data are presented as the average number of fibers (± standard error of the mean [s.e.m.]) per mm2. Signals from intact SYTOX Green-positive cells were excluded from this analysis, and only those exceeding 20 μm in length were considered NET-like formations. The number of neutrophils was quantified using reflected light oblique transillumination in the area 75 μm to each side of the vessel over a 100-μm vessel length (1.5 × 10− 4 mm2).
3
Neutrophil activation and signaling
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Lipopolysaccharide (LPS) from E. coli 055:B5, phorbol 12-myristate 13-acetate (PMA), CXCL8, dibutyryl cyclic AMP (db-cAMP), wortmannin, staurosporine, bovine serum albumin (BSA), and anti-BSA antibody were from Sigma Aldrich (St. Louis, MO, USA); heat-inactivated fetal bovine serum (FBS) was from Gemini-Bioproducts (West Sacramento, CA, USA); misoprostol, LTB4, and PAF were from Cayman Chemical (Ann Arbor, MI, USA); equine recombinant granulocyte-monocyte colony-stimulating factor (GM-CSF) was from Kingfisher Biotech (Saint Paul, MN, USA); and Hank’s balanced salt solution (HBSS) was from Thermo Fisher Scientific (Grand Island, NY, USA).
4
Generating BSA-anti-BSA Immune Complexes
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To generate BSA-anti-BSA immune complexes (IC), anti-BSA antibody (Sigma-Aldrich, catalog number SAB4301142) was diluted to 0.4 mg/ml in PBS and 5 ul 10 mg/ml BSA was added to 750 ul anti- BSA and incubated at 37 °C with minimal shaking for 1 h. After the incubation, the IC were washed in sterile phagocytosis buffer. A BCA was performed on the IC to determine concentration. One-well Lab-Tek chamber slides were coated for 2 h with 2 ml of 4–8 μg/ml HSA or C1q, then gently rinsed twice with sterile phosphate buffered saline (PBS) before the addition of 106 BMDM suspended in phagocytosis buffer. BMDM were adhered for 30 min on HSA or C1q before the addition of 15 μg/mL of IC for the indicated time. In some experiments 300 ng/ml LPS was added for an additional 4 h to detect cytokine production.
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