Alpha lactose

Manufactured by Merck Group

Sourced in United States

Alpha-lactose is a monosaccharide that is commonly used in laboratory applications. It is the primary sugar found in mammalian milk and serves as a key component in various biochemical processes. This product provides a reliable and consistent source of this essential sugar for use in research and analytical settings.

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Lab products found in correlation

Gdp l fucose, by Merck Group (1 mentions) Glycosyltransferase activity kit, by R&D Systems (1 mentions) Rat anti mouse tim 3 antibody clone rmt3 23, by BioXCell (1 mentions)

2 protocols using alpha lactose

Enzymatic Activities of FUT2 and FUT8

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The FUT2 and FUT8 enzyme activities were measured using the Glycosyltransferase activity kit (R&D system, MN, USA) according to the manufacturer’s instructions. The kit utilizes a colorimetric assay specific for phosphate-coupled glycosyltransferase reactions to assay the enzyme activities. The activity of FUT2 in the cell lysate was measured by its ability to transfer fucose from GDP-L-Fucose to alpha-lactose (Sigma-Aldrich, MO, USA). The activity of FUT8 in the cell lysate was measured by its ability to hydrolyze the donor substrate GDP-L-Fucose (Santa Cruz, CA, USA). The glycosyltransferase activity was expressed as phosphate concentration equivalents produced per minute (M P/min).

Huang H., He Y., Li Y., Gu M., Wu M, & Ji L. (2022). Eriodictyol suppresses the malignant progression of colorectal cancer by downregulating tissue specific transplantation antigen P35B (TSTA3) expression to restrain fucosylation. Bioengineered, 13(3), 5551-5563.

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Blocking LAG-3, TIM-3, and PD-1 in HMPV

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To block LAG-3 signaling, WT or PD-1^−/− mice were injected i.p. with 200µg of rat anti-mouse LAG-3 antibody (clone C9B7W, Bio X-cell) on day 3 p.i. On days 5, 7 and 9 p.i., 100µg of anti-LAG-3 or isotype control antibody was given i.n. and 100µg were given i.p. To block TIM-3 and PD-1 signaling concurrently, mice were injected i.p. with 200µg of rat anti-mouse TIM-3 antibody (clone RMT3-23, Bio X-cell) and anti-PD-L1 (clone 10F.9G2) for 2 days prior to infection and then every other day during HMPV infection. To block galectin-9 (Gal-9) binding to TIM-3, WT mice were infected intranasally with HMPV-A2. At day 3 of infection and every day thereafter until euthanasia, mice were injected 2x/day (morning and night) with 277 mM sterile filtered alpha-lactose (Sigma) in PBS, or sterile PBS for controls. To block TIM-3 signaling, PD-1^−/− mice were injected i.p. with 200µg of rat anti-mouse TIM-3 antibody (clone RMT3-23, Bio X-cell) or isotype control antibody on days 3, 5, 7, and 9 post-infection.

Erickson J.J., Rogers M.C., Tollefson S.J., Boyd K.L, & Williams J.V. (2016). Multiple inhibitory pathways contribute to lung CD8+ T cell impairment and protect against immunopathology during acute viral respiratory infection. Journal of immunology (Baltimore, Md. : 1950), 197(1), 233-243.

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