Wizard2tm

Prostate carcinoma cell lines PC-3 (GRPR positive), LNCaP (PSMA positive), and prostate carcinoma PC-3 cells transfected with PSMA PC-3pip (GRPR and PSMA positive) were maintained as described in Appendix A. All animal studies were approved by the Ethics Committee for Animal Research in Uppsala, Sweden following the national legislation on protection of laboratory animals (5/16, 26 February 2016). The activity content was measured using a 2840 automated gamma counter Wizard2TM (PerkinElmer, Norwalk, CT, USA). Statistical analyses were done by unpaired, two-tailed t-tests using GraphPad Prism 8 for Windows (GraphPad Software Inc, San Diego, CA, USA), p values below 0.05 were considered significant.

Prostate carcinoma cell line PC-3 (GRPR positive) was purchased from ATCC, Manassas, VA, USA. The cells were maintained in RPMI-1640 media supplemented with 10% fetal bovine serum (FBS), 1% penicillin–streptomycin (PEST) (100 IU/mL penicillin, 100 µg/mL streptomycin), and 1% 2 mM L-glutamine (L-Glut), all purchased from Biochrom AG, Berlin, Germany, and incubated at 37 °C in 5% carbon dioxide gas and 95% air.
The activity content was measured using a 2840 automated gamma counter Wizard^2TM (PerkinElmer, Waltham, MA, USA). Instant thin-layer chromatography (ITLC) results were analyzed using Cyclone^® Plus (PerkinElmer). Statistical analyses were performed by unpaired, two-tailed t-tests using GraphPad Prism 8 for Windows (GraphPad Software, San Diego, CA, USA); p values below 0.05 were considered significant.