Ml277

Phenylephrine hydrochloride, sodium nitroprusside (SNP), ANP (127–150 rat), XE991 (10,10-bis(4-pyridinylmethyl)-9(10H)-anthracenone dihydrochloride), carbachol chloride, isoprenaline hydrochloride, forskolin, mefenamic acid, diclofenac sodium, levcromakalim and retigabine were sourced from Sigma-Aldrich. Linopirdine hydrochloride, HMR1556 (N-[(3R,4S)-3,4-Dihydro-3-hydroxy-2,2-dimethyl-6-(4,4,4-trifluorobutoxy)-2H-1-benzopyran-4-yl]-N-methylmetanesulfonamide), treprostinil, R-L3 (5-(2-Fluorophenyl)-1,3-dihydro-3-(1H-indol-3-ylmethyl)-1-methyl-2H-1,4-benzodiazepin-2-one), ML277 ((2R)-N-[4-(4-Methoxyphenyl)-2-thiazolyl]-1-[(4-methylphenyl)sulfonyl]-2-piperidinecarboxamide), ICA-069673 (N-(2-Chloro-5-pyrimidinyl)-3,4-difluorobenzamide), GW0742, SC-51322 and iberiotoxin were obtained from Tocris Bioscience (Bristol, UK). Sildenafil citrate was supplied by Cambridge Bioscience (Cambridge, UK), A23187 was from Calbiochem (Beeston, Nottinghamshire, UK) and glyceryl trinitrate (GTN) from Aspire Pharma Ltd. (Petersfield, UK). Depending on solubility, stock solutions were prepared as 100 or 10 mM drug in water or dimethyl sulphoxide (DMSO) and stored as frozen aliquots. DMSO was present at <0.1% in the myograph chamber.