Nude BALB/C mice were purchased from Jackson laboratories (Bar Harbor, ME, USA). DAKO EnVision+ system kits (K4007, K4011) were purchased from Agilent technologies (Carpinteria, CA, USA). All animal studies were conducted in accordance with National Institutes of Health animal use guidelines, and a protocol approved by the University of Louisville’s Institutional Animal Care and Use Committee (IACUC).
Rabbit polyclonal anti-MnSOD (06-984) was purchased from Upstate antibodies (Millipore, Temecula, CA, USA). Mouse monoclonal anti-NF-κB (sc-71677) was purchased from Santa Cruz biotechnology (Dallas, TX, USA). Rabbit polyclonal anti-Nkrf (HPA001476) was purchased from Sigma Aldrich (St. Louis, MO, USA).
Rabbit polyclonal anti-MnSOD (06-984) was purchased from Upstate antibodies (Millipore, Temecula, CA, USA). Mouse monoclonal anti-NF-κB (sc-71677) was purchased from Santa Cruz biotechnology (Dallas, TX, USA). Rabbit polyclonal anti-Nkrf (HPA001476) was purchased from Sigma Aldrich (St. Louis, MO, USA).