Total protein was isolated from cell lysates by using RIPA buffer and quantified by the BCA protein assay kit (Beyotime, Shanghai, China). Proteins were resolved on 10% SDS-PAGE and then transferred to PVDF (Bio-Rad) membranes. After blocking, the membranes were incubated with primary antibodies at 4°C overnight. Then, the membranes were incubated with secondary anti-rabbit antibody (Abcam; 1:5,000) at room temperature for 1 h. Membranes were scanned by using an Odyssey Imaging System and analyzed with Odyssey v2.0 software (LICOR Biosciences, Lincoln, NE, USA). The primary antibodies used in this study are as follows: anti-NCAPG2 (Abcam, Cambridge, MA, USA; 1:1,000), anti-Bax (Abcam; 1:1,000), anti-XIAP (Abcam; 1:1,000), anti-activated caspase 9 (Abcam; 1:1,000), anti-E-cadherin (Abcam; 1:1,000), anti-N-cadherin (Abcam; 1:1,000), anti-Vimentin (Abcam; 1:1,000), and anti-β-actin (Abcam; 1:1,000). β-actin was used as an internal control.
Anti activated caspase 9
Manufactured by Abcam
Sourced in United States
Anti-activated caspase 9 is a lab equipment product that detects the active form of caspase 9, a key enzyme involved in the apoptosis (programmed cell death) pathway. This product is designed to provide a reliable and specific means of measuring caspase 9 activation in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti xiap, by Abcam (1 mentions)
Odyssey v2, by LI COR (1 mentions)
Odyssey imaging system, by LI COR (1 mentions)
Anti n cadherin, by Abcam (1 mentions)
Polyvinylidene fluoride (pvdf), by Bio-Rad (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
Anti β actin, by Abcam (1 mentions)
Anti e cadherin, by Abcam (1 mentions)
Anti bax, by Abcam (1 mentions)
Anti vimentin, by Abcam (1 mentions)
Ecl reagent, by Cell Signaling Technology (1 mentions)
2 protocols using anti activated caspase 9
1
Western Blot Analysis of Protein Expression
2
Quantitative Analysis of Apoptotic Markers via Western Blotting
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Western blotting was performed as described previously (16 (link),17 (link)). After sample preparation, 50 µg protein of each sample was loaded onto an 12% SDS-PAGE gel. After electrophoresis and transfer of proteins to a nitrocellulose membrane, the membranes were blocked at room temperature and incubated for 15 h at 4°C with the following primary antibodies: Anti-BDNF (1:1,000; cat. no. ab108319), anti-Bcl-2 (1:1,000; cat. no. ab59348), anti-Bax (1:1,000; cat. no. ab32503) and anti-activated caspase-3 (1:1,000; cat. no. ab2302), all from Abcam; anti-activated caspase-9 (1:1,000; cat. no. 9507) and anti-β-actin (1:1,000; cat. no. 4970), both from Cell Signaling Technology, Inc. Anti-rabbit or mouse IgG HRP-linked antibodies (all, 1:4,000; cat. no. 7074 and cat. no. 7076, respectively; Cell Signaling Technology, Inc.) were selected to incubate with the membrane at room temperature for 2 h. The blots were visualized with ECL reagent (cat. no. 32106; Thermo Fisher Scientific, Inc.). The protein bands were selected to perform densitometry quantification with ImageJ software (v1.8; National Institutes of Health).
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