Streptozotocin (STZ) and oxidative stress chemicals were procured from Sigma-Aldrich, USA through Bayoni Trading Company KSA. The assay kits such as glucose, aspartate aminotransferase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), and acid phosphatase (ACP) were procured from Crescent Diagnostics, Jeddah, Saudi Arabia. Proinflammatory cytokine assay kits (IL-1β, IL-2, and TNF-α) and apoptosis assay kits (caspase-3 and caspase-9) were purchased from Abcam, USA, through local suplier of Saudi Arabia.
Caspase 3 and caspase 9
Manufactured by Abcam
Sourced in United States, United Kingdom
Caspase-3 and caspase-9 are proteases involved in the apoptosis (programmed cell death) pathway. Caspase-3 is a key effector caspase, while caspase-9 is an initiator caspase. These enzymes play critical roles in the execution and regulation of apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using caspase 3 and caspase 9
1
Streptozotocin-Induced Oxidative Stress
2
Investigating HL60 Cell Apoptosis Pathways
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The human promyelocytic leukemia cell line (HL60) was obtained from the American Type Culture Collection (ATCC). HL60 was subcultured in a 75-cm tissue culture flask with RPMI 1640 media mixed with 10% bovine serum albumin and 1% penicillin-streptomycin (Nacalai Tesque, Inc., Japan). The subculture was then kept in an incubator at 5% CO 2 saturation and 37°C temperature. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was obtained from Nacalai, USA. The antibodies used for western blot, caspase-3 and caspase-9, cytochrome c, β-actin, and secondary antibodies were obtained from Abcam (UK). p53 was purchased from Novus Biologicals (USA) and cleaved caspase-3 and caspase-9 from GeneTex, Inc. (USA). Caspase activity kits were obtained from R&D Systems (USA). The BD detector and Ion-Trap time-of-flight (TOF) mass spectrometer (Shimadzu, Japan). Flow rate: 0.50 mL/min. Column temperature: 40°C. Gradient: 10%-100% MeCN over 7 min. Column: Water Xbridge 50 × 2.1 mm, 2.5 µM. Mobile phase: H 2 O (0.1% formic acid):MeCN (0.1% formic acid); more details have been reported in one of our group study. 17
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