Monoclonal mouse anti hsc70

Manufactured by Santa Cruz Biotechnology

Monoclonal mouse anti-HSC70 is a laboratory reagent used to detect the presence of the heat shock cognate 70 kDa protein (HSC70) in biological samples. HSC70 is a constitutively expressed member of the heat shock protein 70 family, which plays a role in protein folding and trafficking. This antibody can be used in various immunodetection techniques to identify and quantify HSC70 levels in cell lysates, tissue homogenates, or other biological preparations.

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Lab products found in correlation

Monoclonal mouse anti neuronal nuclei neun, by Merck Group (1 mentions) Odyssey clx imaging system, by LI COR (1 mentions)

Close spelling variants of this product

HSC70 (90 citations) Anti-HSC70 (47 citations) Mouse anti-HSC70 (24 citations) Mouse HSC70 (3 citations) Monoclonal mouse anti (2 citations) Anti-HSC70 mouse monoclonal antibody (2 citations) Monoclonal mouse anti-HSC70 antibody (sc-7298) (2 citations)

2 protocols using monoclonal mouse anti hsc70

Immunohistochemistry Antibody Panel

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The following antibodies were used: polyclonal goat anti-Calbindin D28 K (Santa Cruz Biotechnology Inc., Santa Cruz, CA), monoclonal mouse anti-Neuronal Nuclei (NeuN) (Millipore, Billerica, MA), polyclonal rabbit anti GABA(A) receptor alpha 6 subunit receptor (Millipore, Temecula, CA), monoclonal mouse anti-pS139-H2AX (Merck Millipore, Darmstadt, Germany), polyclonal rabbit anti-53BP1 (Novus Biologicals, Littleton CO), monoclonal rabbit anti-ATM D2E2 (Cell Signaling, Danvers, MA), polyclonal rabbit anti-pS824-KAP-1 (Bethyl Laboratories, Montgomery, TX), monoclonal mouse anti-KAP-1 (BD Transduction Laboratories, San Jose, CA), monoclonal mouse anti-HSC70 (Santa Cruz Biotechnology Inc., Santa Cruz, CA).

Tal E., Alfo M., Zha S., Barzilai A., De Zeeuw C.I., Ziv Y, & Shiloh Y. (2018). Inactive Atm abrogates DSB repair in mouse cerebellum more than does Atm loss, without causing a neurological phenotype. DNA repair, 72, 10-17.

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Immunoblotting of Phosphorylated STAT5

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Immunoblotting was performed using standard protocols. The following antibodies were used: polyclonal rabbit anti-phospho-STAT5 (Y694) (#71-6900; Invitrogen, Thermo Fisher Scientific; 1:1000), monoclonal mouse anti-STAT5 (#610191; BD Biosciences; 1:1000), monoclonal mouse anti-HSC70 (#sc-7298; Santa Cruz Biotechnology; 1:10000) and monoclonal mouse anti-α-tubulin (#sc-32293; Santa Cruz Biotechnology; 1:5000). Images of membranes were obtained using IRDye fluorescent secondary antibodies and an Odyssey CLx imaging system (LI-COR).

de Araujo E.D., Erdogan F., Neubauer H.A., Meneksedag-Erol D., Manaswiyoungkul P., Eram M.S., Seo H.S., Qadree A.K., Israelian J., Orlova A., Suske T., Pham H.T., Boersma A., Tangermann S., Kenner L., Rülicke T., Dong A., Ravichandran M., Brown P.J., Audette G.F., Rauscher S., Dhe-Paganon S., Moriggl R, & Gunning P.T. (2019). Structural and functional consequences of the STAT5BN642H driver mutation. Nature Communications, 10, 2517.

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