Hrp labeled donkey anti goat antibody

Manufactured by Santa Cruz Biotechnology

The HRP-labeled donkey anti-goat antibody is a secondary antibody used in various immunoassays and immunohistochemical techniques. It is produced by immunizing donkeys with goat IgG and labeling the resulting antibodies with horseradish peroxidase (HRP). The HRP label allows for the detection and visualization of target antigens recognized by the primary goat antibody.

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Lab products found in correlation

Anti tubulin antibody, by Merck Group (1 mentions) Anti bzlf1 antibody, by Santa Cruz Biotechnology (1 mentions) Anti phosphorylated yap s127, by Cell Signaling Technology (1 mentions) Anti lats1 antibody, by Cell Signaling Technology (1 mentions) Anti β actin antibody, by Merck Group (1 mentions) Anti hsp90 f 8, by Santa Cruz Biotechnology (1 mentions) Anti taz, by Novus Biologicals (1 mentions) Anti bmrf1, by Merck Group (1 mentions) Anti p18, by Thermo Fisher Scientific (1 mentions) Anti involucrin, by Merck Group (1 mentions) Anti blimp1, by Cell Signaling Technology (1 mentions) Anti δnp63, by BioLegend (1 mentions) Anti klf4, by Cell Signaling Technology (1 mentions) Hrp labeled goat anti mouse antibody, by Thermo Fisher Scientific (1 mentions) Anti bzlf1, by Santa Cruz Biotechnology (1 mentions) Anti c myc, by Abcam (1 mentions)

2 protocols using hrp labeled donkey anti goat antibody

Dissecting YAP/TAZ Signaling Pathways

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Anti-YAP/TAZ (D24E4) dual antibodies were used at 1:1000 (Cell Signaling Technologies, catalog# 8418S), anti-YAP (D8H1X) antibody used at 1:1000 (Cell Signaling Technologies catalog# 14074T), anti-phosphorylated YAP S127 at 1:1000 (Cell Signaling Technologies, catalog# 4911), anti-TAZ (Novus, catalog# NB110-58359), anti-phosphorylated TAZ S89 (E1X9C) at 1:1000 (Cell Signaling Technologies, catalog# 59971), anti-BZLF1 antibody was used at 1:500 (Santa Cruz, catalog# sc-53904), anti-BRLF1 antibody at 1:2000 (isolated from rabbits injected with peptide sequence EDPDEETSSQAVKALREMAD), anti-BMRF1 antibody at 1:2000 (Millipore-Sigma, catalog# MAB8186), anti-LATS1 antibody at 1:1000 (Cell Signaling Technologies, catalog# 9153), anti-phosphorylated LATS S909 at 1:1000 (Cell Signaling Technologies catalog# 9157), PAN-TEAD antibodies at 1:1000 (Cell Signaling Technologies catalog# 13295S), anti-tubulin antibody at 1:4000 (Sigma, catalog# T5168), anti-β actin antibody at 1:5000 (Sigma catalog# 5441), and anti-HSP90 (F8) at 1:1000 (Santa Cruz catalog# sc-13119). The secondary antibodies used were Horseradish peroxide (HRP)- labeled goat anti-mouse antibody at 1:5000 (Thermo Scientific catalog# 31430), HRP- labeled donkey anti-goat antibody (Santa Cruz catalog# sc-2056, 1:5000), and HRP- labeled anti-rabbit antibody (Fisher Scientific catalog# 31460 1:10000).

Van Sciver N., Ohashi M., Pauly N.P., Bristol J.A., Nelson S.E., Johannsen E.C, & Kenney S.C. (2021). Hippo signaling effectors YAP and TAZ induce Epstein-Barr Virus (EBV) lytic reactivation through TEADs in epithelial cells. PLoS Pathogens, 17(8), e1009783.

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Detailed Antibody Use for Immunoblot Analysis

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The following antibodies were used for immunoblot analyses in this study: anti-R rabbit polyclonal antibody directed against the R peptide (peptide sequence EDPDEETSSQAVKALREMAD 1: 2500), anti-p63ɑ (Cell Signaling Technologies catalog # 13109 1:1000), anti-ΔNp63 (Biolegend, catalog #619001), anti-KLF4 (Cell Signaling Technologies, catalog #4038 1: 1000), anti-BZLF1 (Santa Cruz, catalog # sc-53904, 1:500), anti-BMRF1 (Millipore, catalog # MAB8186, 1: 2500), anti-p18 (Thermo-Fisher Scientific, # PA1-73003, 1:2000), anti-BLIMP1 (Cell Signaling Technologies catalog # 9115S 1:1000), anti-involucrin (Sigma, catalog #19018, 1:3000), anti-c-myc (Abcam catalog# ab32072, 1:10000), p38 MAPK14 antibody (Cell Signaling Technologies catalog#9218 1:1000) and phospho-p38 MAPK (Cell Signaling Technologies catalog #9211 1:1000), anti-EBV SM polyclonal rabbit antibody (1:750), a generous gift from Sankar Swaminathan of the University of Utah, anti-Tristetraprolin (Cell Signaling Technologies catalog# #71632 1:1000). The secondary antibodies used were Horseradish peroxide (HRP)- labeled goat anti-mouse antibody (Thermo Scientific# 31430, 1:5000), HRP- labeled donkey anti-goat antibody (Santa Cruz#sc-2056, 1:5000), and HRP- labeled goat anti-rabbit antibody (Fisher Scientific 1:10000).

Van Sciver N., Ohashi M., Nawandar D.M., Pauly N.P., Lee D., Makielski K.R., Bristol J.A., Tsao S.W., Lambert P.F., Johannsen E.C, & Kenney S.C. (2021). ΔNp63α promotes Epstein-Barr virus latency in undifferentiated epithelial cells. PLoS Pathogens, 17(11), e1010045.

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