Paclitaxel (PTX) was obtained from Samyang Biopharmaceuticals Co. (Seoul, Korea). Human serum albumin (HSA) and nile red were purchased from Sigma-Aldrich (St. Louis, MO). Methoxy-poly(ethyleneglycol) succimidylglutarate (mPEG-NHS, MW 5000) was purchased from SunBio Inc. (Anyang, Korea). Cell culture reagents, including Dulbecco’s modified Eagle’s medium (DMEM), phosphate-buffered saline (PBS, pH 7.4), and trypsin-EDTA, and fluorescent dyes for confocal microscopic imaging, including 4′,6-diamidino-2-phenylindole (DAPI) and 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiIC18), were purchased from Invitrogen (Carlsbad, CA). Abraxane® was purchased from Celgene Co. (Summit, NJ). All organic solvents were of analytical grade and were used without further purification.
1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanine perchlorate diic18
Manufactured by Thermo Fisher Scientific
Sourced in United States, Japan
1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiIC18) is a fluorescent dye used for labeling and tracing lipid components in biological membranes.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (2 mentions)
Human serum albumin (hsa), by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Nile red, by Merck Group (1 mentions)
Cleancap egfp mrna 5 mou, by TriLink (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Sodium acetate, by Merck Group (1 mentions)
Cholesterol, by Merck Group (1 mentions)
Anhydrous dimethyl sulfoxide, by Merck Group (1 mentions)
Alexa fluor 555 conjugate, by Thermo Fisher Scientific (1 mentions)
1 palmitoyl 2 oleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions)
1 palmitoyl 2 oleoyl sn glycero 3 phospho 1 rac glycerol, by Avanti Polar Lipids (1 mentions)
Triton x 100 tx 100, by Merck Group (1 mentions)
Ganglioside, by Avanti Polar Lipids (1 mentions)
5 protocols using 1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanine perchlorate diic18
1
Paclitaxel-Loaded Albumin Nanoparticle Synthesis
2
Lipid-based mRNA Delivery Protocol
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The lipids DSPC and ESM were purchased from Avanti Polar Lipids (Alabaster, AL). The ionizable cationic lipid heptatriaconta-6,9,28,31-tetraen-19-yl 4-(dimethylamino)butanoate (DLin-MC3-DMA; MC3) was synthesized by Biofine International (Vancouver, BC, Canada). Cholesterol, sodium acetate, Dulbecco’s phosphate-buffered saline (PBS), FBS, and Triton X-100 were purchased from Sigma-Aldrich (St. Louis, MO). (R)-2,3-bis(octadecyloxy)propyl-1-(methoxy polyethylene glycol 2000) carbamate (PEG-DMG) was provided by Alnylam Pharmaceuticals. Lipid tracers 1,1′-dioctadecyl- 3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI-C18) and 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate Salt (DiD-C18) were purchased from Invitrogen (Burlington, ON). Dulbecco’s modified Eagle’s medium (DMEM) was purchased from Thermo Fischer Scientific. Luciferase mRNA was provided by Dr. Drew Weismann’s lab (University of Pennsylvania). CleanCap EGFP mRNA (5moU) was purchased from TriLink Biotechnologies (San Diego, CA).
3
Fluorescently Labeled Lipid Membrane Characterization
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The lipids 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), and cholesterol (Chol) were used in this work. Head group–labeled rhodamine dye 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (14:0 Liss Rhod PE) was used as the fluorophore to label supported lipid bilayers. The lipids and dye were purchased from Avanti Polar Lipids (Alabama) and dissolved in chloroform.
1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI-C18, #D3911), octadecyl rhodamine B chloride (#O246), and CTxB-555 (cholera toxin subunit B [recombinant] with Alexa Fluor 555 conjugate, #C34776) were purchased from Invitrogen (Thermo Fisher Scientific, Singapore). They were dissolved in anhydrous dimethyl sulfoxide (#276855, Sigma-Aldrich, Singapore) to prepare the stock solutions. The fluidizer 1-heptanol 98% (#H2805) was purchased from Sigma-Aldrich (Singapore).
An Alexa Fluor 488–conjugated EGFR monoclonal antibody was purchased from Cell Signaling Technology (EGF Receptor [D38B1] XP Rabbit mAb [Alexa Fluor 488 Conjugate], #5616S, MA).
1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI-C18, #D3911), octadecyl rhodamine B chloride (#O246), and CTxB-555 (cholera toxin subunit B [recombinant] with Alexa Fluor 555 conjugate, #C34776) were purchased from Invitrogen (Thermo Fisher Scientific, Singapore). They were dissolved in anhydrous dimethyl sulfoxide (#276855, Sigma-Aldrich, Singapore) to prepare the stock solutions. The fluidizer 1-heptanol 98% (#H2805) was purchased from Sigma-Aldrich (Singapore).
An Alexa Fluor 488–conjugated EGFR monoclonal antibody was purchased from Cell Signaling Technology (EGF Receptor [D38B1] XP Rabbit mAb [Alexa Fluor 488 Conjugate], #5616S, MA).
4
Liposomal Bisphenol A Synthesis
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Dipalmitoylphosphatidylcholine (DPPC), cholesterol and methoxy‐polyethyleneglycol (2000)‐conjugated distearoylphosphatidylethanolamine (DSPE‐MPEG) were gifts from Nippon Fine Chemical (Takasago, Hyogo, Japan). DSPE‐PEG‐NHS (N‐hydroxysuccinimide) was purchased from NOF (Tokyo, Japan). 1,1′‐Dioctadecyl‐3,3,3′,3′‐tetramethylindocarbocyanine perchlorate (DiIC18) was obtained from Life Technologies Japan (Tokyo, Japan). BPA, FITC‐conjugated BPA (BPA‐FITC) and biotin‐conjugated BPA were kindly donated by J‐OIL MILLS (Tokyo, Japan).
5
Lipid Fluorescent Probe Preparation
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The lipids 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol), sodium salt (POPG), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-dioleoyl-3-trimethylammonium-propane, chloride salt (DOTAP), egg (chicken) sphingomyelin (SM), Ganglioside (Ovine Brain) (GM1) and cholesterol and the fluorescent probes 1-palmitoyl-2-6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl}-sn-glycero-3-phosphoethanolamine (NBD-PE) and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (DPPE-Rh) were purchased from Avanti Polar Lipids (Alabaster, AL). The fluorescent probe 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindocarbocyanine Perchlorate (DiI C18) was obtained from Life Technologies (Carlsbad, CA). Low-melting temperature agarose was obtained from Fischer Scientifics (Walthan, MA) and Cholera Toxin Subunit B (Recombinant) Alexa Fluor 488 Conjugate (CTB-Alexa) was purchased from Invitrogen (Carlsbad, CA). The fluorescent dye sulforhodamine B (SRB), carboxyfluorescein (CF), the detergent Triton X-100 (TX-100) and all other chemicals were purchased from Sigma Aldrich (St. Louis, MO). All chemicals were used without further purification. Milli-Q water was used throughout the work.
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