Rtca celligence system

Adherent target cells (CHO or CHO-BCMA) were seeded into 96-well E-plates (Acea Biosciences, San Diego, CA, USA) at 1 × 10⁴ cells per well and monitored in culture overnight with the impedance-based real-time cell analysis (RTCA) × CELLigence system (Acea Biosciences). The next day, the medium was removed and replaced with AIM V-AlbuMAX medium containing 10% FBS ± 1 × 10⁵ effector cells (CAR-T cells, non-transduced T cells, or T cells with BCMA-CD3 bispecific antibodies), in triplicate. The cells in the E-plates were monitored for another one to two days with the RTCA system, and impedance was plotted over time.

Adherent target cells (CHO-CS1; CHO; Hela-CS1 or Hela) (1 × 10⁴ cells per well) were seeded into 96-well E-plates (Acea Biosciences, San Diego, CA, USA) using the impedance-based real-time cell analysis (RTCA) × CELLigence system (Acea Biosciences, San Diego, CA, USA). The next day, the medium was removed and replaced with AIM V-AlbuMAX medium containing 10% FBS ± 1 × 10⁵ effector cells in triplicate (CAR-T cells or non-transduced T cells). The cells were monitored for another 24–48 h with the RTCA system, and impedance was plotted over time. Cytolysis was calculated as (impedance of target cells without effector cells minus impedance of target cells with effector cells) × 100/impedance of target cells without effector cells.