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Phospho kit tyr719

Manufactured by Cell Signaling Technology
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Phospho-KIT (Tyr719) is a primary antibody that specifically detects KIT protein phosphorylated at tyrosine 719. It is designed for use in various immunoassay applications to study KIT signaling pathways.

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Lab products found in correlation

Imatinib, by Selleck Chemicals (1 mentions) Sorafenib, by Selleck Chemicals (1 mentions) Sunitinib, by Selleck Chemicals (1 mentions) Dasatinib, by Selleck Chemicals (1 mentions) Nilotinib, by Selleck Chemicals (1 mentions) Ponatinib, by Selleck Chemicals (1 mentions) Ab15580, by Abcam (1 mentions) Cck 8 kit, by Selleck Chemicals (1 mentions) Axitinib, by Selleck Chemicals (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions) Anti gapdh 14c10, by Cell Signaling Technology (1 mentions) Phospho kit tyr703, by Cell Signaling Technology (1 mentions) Flt3 flk 2 c 20 sc 479, by Santa Cruz Biotechnology (1 mentions)

2 protocols using phospho kit tyr719

1

Tyrosine Kinase Inhibitor Screening

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Axitinib, imatinib, dasatinib, ponatinib, sorafenib, sunitinib, and nilotinib were purchased from Selleck Chemical Inc (Houston, TX, USA). The following commercially available antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA): KIT (#3074), phospho-KIT (Tyr719, #3391), AKT (#4685), phospho-AKT (Ser474, #4060), ERK1/2 (#4695), phospho-ERK1/2 (Tyr202/Tyr204, #4370), and GAPDH (#2118). Rabbit polyclonal antibody against human Ki67 (ab15580) was purchased from Abcam (Cambridge, UK). Rabbit polyclonal antibody against human S-100A (catalog, GZ031129), mouse polyclonal antibodies against human Melan-A (catalog, GM719629) and HMB-45(catalog, GM063429) were purchased from GeneTech company limuted (shanghai, China). Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) KIT and CCK-8 KIT were purchased from Bimake (Houston, TX, USA).
Han Y., Gu Z., Wu J., Huang X., Zhou R., Shi C., Tao W., Wang L., Wang Y., Zhou G., Li J., Zhang Z, & Sun S. (2019). Repurposing Ponatinib as a Potent Agent against KIT Mutant Melanomas. Theranostics, 9(7), 1952-1964.
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2

Western Blot Analysis of Tyrosine Kinases

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Anti-GAPDH (14C10) (rabbit mAb, #2118) (Cell Signaling Technology (Danvers, MA)) was used at a dilution of 1:3000. Also purchased from Cell Signaling Technology were phospho-KIT (Tyr719) (rabbit Ab, #3391), phospho-KIT (Tyr703) (rabbit Ab, #3073), KIT (D13A2) XP (rabbit mAb, #3074), and phospho-FLT3 (Tyr591) (54H1) (mouse mAb, #3466); each was used at a dilution of 1:1000. FLT3/Flk-2 (C-20) (sc-479) (Santa Cruz Biotechnology, Inc. (Dallas, TX)) was used at a dilution of 1:1000, and p-TYR (4G10), #05-321 (MilliporeSigma (Burlington, MA)) was used at a dilution of 1:1000.
Weisberg E., Meng C., Case A., Sattler M., Tiv H.L., Gokhale P.C., Buhrlage S., Liu X., Yang J., Wang J., Gray N., Stone R., Adamia S., Dubreuil P., Letard S, & Griffin J.D. (2019). Comparison of effects of midostaurin, crenolanib, quizartinib, gilteritinib, sorafenib and BLU-285 on oncogenic mutants of KIT and FLT3 in hematologic malignancies: Mutant KIT and FLT3 response to Tyrosine Kinase Inhibitors. British journal of haematology, 187(4), 488-501.
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