For the invasion assays, transwell migration chambers and Matrigel-coated chambers (Becton Dickinson, Waltham, MA) were used. In summary, 5 × 104 HCC cells were seeded into the upper chamber in a serum-free culture medium. The lower chamber was filled with 5 × 104 TCs in DME medium(Thermo Fisher, HyClone, UT, USA) with 10% FBS(Thermo Fisher Scientific, MA, USA). After 48 h of incubation, cells that crossed the membrane were stained with 1% crystal violet and quantified using a microscope (CKX-51, OLYMPUS company, Japan).
Dme medium
Manufactured by Thermo Fisher Scientific
Sourced in United States
DME medium is a cell culture medium designed for the growth and maintenance of various cell types. It provides the necessary nutrients and components to support cell proliferation and viability. The core function of DME medium is to serve as a standardized and optimized culture environment for in vitro cell-based assays and experiments.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dme medium
1
Transwell Invasion Assay for Cancer Cells
2
Measuring Metastatic Potential of HCC Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the invasion assays, transwell migration chambers and Matrigel coated chambers (Becton Dickinson, Waltham, MA) were used. In summary, 5 × 10 4 HCC cells were seeded into the upper chamber in a serumfree culture medium. The lower chamber was lled with 5 × 10 4 TCs in DME medium(Thermo Fisher, HyClone, UT, USA) with 10% FBS(Thermo Fisher Scienti c, MA, USA). After 48 h of incubation, cells that crossed the membrane were stained with 1% crystal violet and quanti ed using a microscope (CKX-51, OLYMPUS company, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!