May grunwald solution

Macrophages were also cultured for 7 days with the cytokines in chamber slides as described before. The slides were rapidly dried well in the air, and then fixed by May-Grunwald solution (Muto Pure Chemicals, Tokyo, Japan) for 2 min at RT. Equal volume of phosphate buffer pH 6.4 (Muto Pure Chemicals) was applied on the slides with May-Grunwald solution for 2 min at RT. After a 20-s rinse with Giemsa solution (Muto Pure Chemicals) diluted by phosphate buffer pH 6.4, the slides were incubated with Giemsa solution for 15 min at RT. Following short rinse by distilled water, the samples were rapidly dried well in the air, immersed in xylene, and mounted with the non-aqueous mounting medium DPX (Merck, Darmstadt, Germany).