Five HCC cell lines (HepG2, Hep3B, SNU182, SNU449, MHCC-97H) and CL-48 normal hepatocytes all were purchased from Shanghai Institute of Cell Biology (Shanghai, China) and cultured in RMPI-1640 medium with 3% fetal bovine serum (FBS; Tiangen, Shanghai, China) plus 100 U/ml penicillin and streptomycin (100 µg/ml) purchased from Tiangen. The surgically sectioned HCC specimens were acquired from The First Affiliated Hospital of Wenzhou Medical University (Wenzhou, China) from July 2010 to October 2014. Immediately after surgical resection, these tissues were stored at −80°C until usage. None patients received preoperative chemotherapy or radiotherapy. All patients have signed formal consent forms. The research for human samples was approved by Ethics Committee of The First Affiliated Hospital of Wenzhou Medical University.
Snu449
Manufactured by Shanghai Institute of Biochemistry and Cell Biology
Sourced in China
SNU449 is a hepatocellular carcinoma cell line derived from a 52-year-old male patient. It is commonly used in research related to liver cancer and associated molecular mechanisms.
Automatically generated - may contain errors
Lab products found in correlation
Hepg2, by Shanghai Institute of Biochemistry and Cell Biology (2 mentions)
Penicillin, by Tiangen Biotech (1 mentions)
Streptomycin, by Tiangen Biotech (1 mentions)
Hep3b, by Shanghai Institute of Biochemistry and Cell Biology (1 mentions)
Snu182, by Shanghai Institute of Biochemistry and Cell Biology (1 mentions)
Mhcc 97h, by Shanghai Institute of Biochemistry and Cell Biology (1 mentions)
Sk hep 1, by Shanghai Institute of Biochemistry and Cell Biology (1 mentions)
Huh 7, by Shanghai Institute of Biochemistry and Cell Biology (1 mentions)
Thle 3, by Shanghai Institute of Biochemistry and Cell Biology (1 mentions)
2 protocols using snu449
1
HCC Cell Lines and Tissue Specimens
2
Profiling HCC Tumor Transcriptome
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HCC tumors and adjacent healthy tissues were collected from patients diagnosed with HCC between 2013 and 2016 and they have undergone operation at a hospital. A total of 76 pairs of tissue samples were used for RNA extraction. We got approval of the organizational application in this investigation from the Hospital Ethics Committee (ZUFH-2013127). HCC cell lines HEPG2, HUH7, SNU449 and SK-HEP-1 from Human as well as a normal human liver cell line THLE-3 were purchased from Shanghai Institute of Cell Biology (Shanghai, China) and cultured in RPMI-1640 medium (HyClone, Logan) Cultivate., UT, USA) supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic. The clinicopathological features for HCC patients were described in Supplementary Table 1 . This research has been carried out in accordance with the World Medical Association Declaration of Helsinki and that all subjects provided written were informed consent.
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