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Csu x1

Manufactured by Teledyne

The CSU-X1 is a compact, high-performance laboratory equipment designed for a variety of applications. It features advanced technology and precise control capabilities to support various experimental and analytical tasks. The core function of the CSU-X1 is to provide a reliable and versatile platform for conducting laboratory-based investigations and measurements.

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3 protocols using csu x1

1

Dual-channel Imaging of Transgenic Hydra

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Dual-labeled transgenic Hydra were prepared for imaging as described16 (link). Imaging was performed using a custom dual-channel spinning disc confocal microscope (Solamere Yokogawa CSU-X1) with a sCMOS camera for each channel (Teledyne-Photometrics Prime-BSI). Samples were simultaneously illuminated with both 488 nm and 561 nm lasers (Coherent OBIS). Emission light was split with a dichroic mirror sending green light to one camera and red light to the other. Cameras were aligned with pinholes and images were registered during processing. Images were captured with a frame rate of 10 frames per second using a 6X objective (Navitar HR Plan Apo 6X/0.3) and Micro-Manager software.
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2

Live Imaging of Transgenic Hydra

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Dual-labeled transgenic Hydra were prepared for imaging as described16 (link). Imaging was performed using a custom dual-channel spinning disc confocal microscope (Solamere Yokogawa CSU-X1) with a sCMOS camera for each channel (Teledyne-Photometrics Prime-BSI). Samples were simultaneously illuminated with both 488 nm and 561 nm lasers (Coherent OBIS). Emission light was split with a dichroic mirror sending green light to one camera and red light to the other. Cameras were aligned with pinholes and images were registered during processing. Images were captured with a frame rate of 10 frames per second using a 6X objective (Navitar HR Plan Apo 6X/0.3) and Micro-Manager software.
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3

Spinning Disk Confocal Imaging

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Cells were imaged with a Plan-Apochromat 40x/1.3NA oil objective on a Zeiss CSU Spinning Disk Confocal Microscope with a CSU-X1 Yokogawa spinning disk scan head on a Prime 95B sCMOS camera (Teledyne Photometrics). The 488/561/647nm laser powers were set at 100/200/20µW with 300/300/150ms exposure times, respectively.
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