Synthetic complete drop out medium
Synthetic complete (SC) drop out medium is a type of culture medium used in microbiology and molecular biology laboratories. It is designed to support the growth of yeast and other microorganisms that require specific nutrient supplements for their growth and development.
2 protocols using synthetic complete drop out medium
Yeast Genetic Transformation Protocol
Recombinant Protein Production in E. coli
LB medium for growth of Escherichia coli was supplied from Carl Roth GmbH + Co. KG (Karlsruhe, Germany), and was supplemented with 100 μg/L of ampicillin for amplification of plasmids. Yeast Extract Peptone Dextrose (YPD) medium with 20 g/L glucose was used for growth of wildtype strains prior to transformation. For preparation of pre- and main cultures we used synthetic complete (SC) drop-out medium (Formedium LTD, Hustanton, England), supplied with 6.7 g/L yeast nitrogen base, 20 g/L glucose and all amino acids necessary for the corresponding auxotrophy.
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