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Synthetic complete drop out medium

Manufactured by Formedium
Sourced in United Kingdom

Synthetic complete (SC) drop out medium is a type of culture medium used in microbiology and molecular biology laboratories. It is designed to support the growth of yeast and other microorganisms that require specific nutrient supplements for their growth and development.

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2 protocols using synthetic complete drop out medium

1

Yeast Genetic Transformation Protocol

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l-threonine, 2-ketobutyric acid, (R)- and (S)-aminobutyric acid, (R) and (S)-2-aminobutanol were bought from Sigma-Aldrich Chemie AG (Steinheim, Germany). Yeast Extract Peptone (YPD) medium with 20 g/L glucose was used for growth of the wildtype strain prior to transformation and both bought from Sigma-Aldrich Chemie AG. Synthetic complete (SC) drop out medium (Formedium LTD, Hustanton, England) with 6.7 g/L yeast nitrogen base without amino acids and ammonium sulfate (Sigma-Aldrich Chemie AG), and 20 g/L glucose (Sigma-Aldrich Chemie AG) was used for pre- and main cultures. LB medium for growth of E. coli was supplied from Carl Roth GmbH + Co. KG (Karlsruhe, Germany).
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2

Recombinant Protein Production in E. coli

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All chemicals were bought from Sigma-Aldrich (St. Louis Missouri, USA) unless stated otherwise. Authentic standard of cembrene was purchased from CHEMOS GmbH & Co. KG (Regenstauf, Germany). Authentic standard of casbene was received from Professor Birger Møller's laboratory at the University of Copenhagen.
LB medium for growth of Escherichia coli was supplied from Carl Roth GmbH + Co. KG (Karlsruhe, Germany), and was supplemented with 100 μg/L of ampicillin for amplification of plasmids. Yeast Extract Peptone Dextrose (YPD) medium with 20 g/L glucose was used for growth of wildtype strains prior to transformation. For preparation of pre- and main cultures we used synthetic complete (SC) drop-out medium (Formedium LTD, Hustanton, England), supplied with 6.7 g/L yeast nitrogen base, 20 g/L glucose and all amino acids necessary for the corresponding auxotrophy.
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