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Hpb all

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The HPB-ALL is a specialized laboratory equipment designed for the cultivation and storage of human peripheral blood lymphocytes (HPB-ALL). It provides a controlled environment for the maintenance and growth of these cell lines, which are valuable research tools in the field of immunology and cell biology.

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Lab products found in correlation

Molt 4, by American Type Culture Collection (5 mentions) Kopt k1, by American Type Culture Collection (5 mentions) Jurkat, by American Type Culture Collection (5 mentions) Fetal bovine serum (fbs), by Merck Group (4 mentions) Tall 1, by American Type Culture Collection (4 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (4 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) Ccrf cem, by American Type Culture Collection (3 mentions) Dnd41, by American Type Culture Collection (2 mentions) Sup t13, by American Type Culture Collection (2 mentions) Molt 16, by American Type Culture Collection (2 mentions) Rpmi 8402, by American Type Culture Collection (2 mentions) Loucy, by American Type Culture Collection (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Sup t1, by American Type Culture Collection (1 mentions) Penicillin, by Merck Group (1 mentions) Streptomycin, by Merck Group (1 mentions) Rpmi 1640 medium, by Merck Group (1 mentions) Hil 7, by Miltenyi Biotec (1 mentions) T cell activation expansion kit, by Miltenyi Biotec (1 mentions)

10 protocols using hpb all

1

Establishment and Maintenance of Human T-ALL Cell Lines

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All human T-ALL cell lines (KOPT-K1, DND41, JURKAT, SUP-T13, HPB-ALL, MOLT-4, MOLT-16, RPMI-8402, TALL-1, and LOUCY) were obtained from ATCC (Manassas, VA, USA) or DSMZ (Braunschweig, Germany). The creation of Ba/F3 cells transformed by TYK2-E957D was described previously.6 (link) JURKAT and KOPT-K1 cells overexpressing BCL2 were generated with the MSCV-IRES-GFP retroviral expression system. JURKAT and KOPT-K1 cells overexpressing BCLXL or MCL1 cDNA were generated with the pHAGE-CMV-IRES-ZsGreen lentiviral expression system. For additional information, see Supplementary Materials and Methods. These cells were maintained in RPMI-1640 medium (GIBCO, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO, USA) and 1% penicillin/streptomycin (Invitrogen, Waltham, MA, USA).
Akahane K., Sanda T., Mansour M.R., Radimerski T., DeAngelo D.J., Weinstock D.M, & Look A.T. (2015). HSP90 inhibition leads to degradation of the TYK2 kinase and apoptotic cell death in T-cell acute lymphoblastic leukemia. Leukemia, 30(1), 219-228.
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2

Establishment and Maintenance of Human T-ALL Cell Lines

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All human T-ALL cell lines (KOPT-K1, DND41, JURKAT, SUP-T13, HPB-ALL, MOLT-4, MOLT-16, RPMI-8402, TALL-1, and LOUCY) were obtained from ATCC (Manassas, VA, USA) or DSMZ (Braunschweig, Germany). The creation of Ba/F3 cells transformed by TYK2-E957D was described previously.6 (link) JURKAT and KOPT-K1 cells overexpressing BCL2 were generated with the MSCV-IRES-GFP retroviral expression system. JURKAT and KOPT-K1 cells overexpressing BCLXL or MCL1 cDNA were generated with the pHAGE-CMV-IRES-ZsGreen lentiviral expression system. For additional information, see Supplementary Materials and Methods. These cells were maintained in RPMI-1640 medium (GIBCO, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO, USA) and 1% penicillin/streptomycin (Invitrogen, Waltham, MA, USA).
Akahane K., Sanda T., Mansour M.R., Radimerski T., DeAngelo D.J., Weinstock D.M, & Look A.T. (2015). HSP90 inhibition leads to degradation of the TYK2 kinase and apoptotic cell death in T-cell acute lymphoblastic leukemia. Leukemia, 30(1), 219-228.
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3

T-ALL Cell Line and PDX Generation

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The human leukemia cell lines CCRF-CEM, HPB-ALL, KOPT-K1, LOUCY, MOLT4, and SUP-T1 were purchased from the ATCC (Manassas, VA, USA) or Deutsche Sammlung von Mikroorganismen und Zellkulturen (Braunschweig, Germany). The mouse T-ALL cell lines LPN228 and LPN49 were generated from conditional knockout mice deficient in PTEN [27 (link)]. The cells were maintained in RPMI 1640 medium containing 10% heat-inactivated fetal bovine serum with penicillin and streptomycin (Sigma-Aldrich, St. Louis, MO, USA) at 37 °C with 5% CO2 in a humidified incubator. T-ALL PDX cells CUL76, 6506870 and D115 were previously established and kindly provided Dr. Adolfo Ferrando (Columbia University, New York, NY, USA) and Dr. Marina Konopleva’s Lab (UTMDACC, Houston, TX) respectively [28 (link), 29 (link)].
Piya S., Yang Y., Bhattacharya S., Sharma P., Ma H., Mu H., He H., Ruvolo V., Baran N., Davis R.E., Jain A.K., Konopleava M., Kantarjian H., Andreeff M., You M.J, & Borthakur G. (2022). Targeting the NOTCH1-MYC-CD44 axis in leukemia-initiating cells in T-ALL. Leukemia, 36(5), 1261-1273.
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4

Establishment of Primary Lymphoma Cell Lines

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Primary lymphomas tumors and the lymphoma cell lines were collected and established in our laboratory as described previously between 2004 and 2005 (24 (link)). HPB-ALL, ALL-SIL and 293T cell lines were obtained from the ATCC (Manassas, VA, USA) and cultured under recommended condition for less than six months. Mouse embryonic fibroblasts (MEFs) were prepared from wild-type C57Bl/6 embryos at day E13.5 following a standard MEF isolation protocol (25 ) and were cultured in complete DMEM. Animal experiments were approved by the University of Miami Institutional Animal Care and Use Committee. The number of the passages of the cell lines used in this study are not exceed 15. All cell lines were maintained at 37 °C in 5% CO2 and tested for mycoplasma contamination.
Han X., Ranganathan P., Tzimas C., Weaver K.L., Jin K., Astudillo L., Zhou W., Zhu X., Li B., Robbins D.J, & Capobianco A.J. (2017). Notch Represses Transcription by PRC2 Recruitment to the Ternary Complex. Molecular cancer research : MCR, 15(9), 1173-1183.
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5

Cytotoxicity Evaluation of ADCs in T-cell Malignancies

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Human CTCL cell lines (HH, H9, MJ, Hut 78) and T-cell acute lymphoblastic leukemia (T-ALL) cell lines (HPB-ALL, PF-382, CCRF-CEM, and Jurkat) were acquired from American Type Culture Collection (ATCC) and cultured in complete media recommended by ATCC. All cell lines were passaged 3 times per week and maintained at a cell density below 106 cells/mL, and logarithmically growing cells were used for all experiments.
SGN-CD70A (h1F6239C-PBD) and ADC-IgG control (hIgG239C-PBD) were provided by Seagen Inc. (Seattle, WA).17 (link) T-cell activation/expansion kit and recombinant cytokines, human IL-2 (hIL-2) and hIL-7, were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany).
Wu C.H., Wang L., Yang C.Y., Wen K.W., Hinds B., Gill R., McCormick F., Moasser M., Pincus L, & Ai W.Z. (2022). Targeting CD70 in cutaneous T-cell lymphoma using an antibody-drug conjugate in patient-derived xenograft models. Blood Advances, 6(7), 2290-2302.
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6

Comparing Human and Mouse Lymphoma Cells

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Human T lymphoma cell line HPB-ALL and mouse lymphoma cell line EL4 were obtained from American Type Culture Collection (ATCC, Manassas, VA). 293FT cell line was obtained from ThermoFisher Scientific (Grand Island, NY). Normal human peripheral blood mononuclear cells (PBMC) were obtained from consented healthy donors in Augusta Shepard Community bank. All studies with human samples were approved by Augusta University Institutional Review Board.
Liu M.Y., Klement J.D., Langan C.J., van Riggelen J, & Liu K. (2021). Expression regulation and function of PD-1 and PD-L1 in T lymphoma cells. Cellular immunology, 366, 104397.
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7

Cell Line Authentication and Maintenance

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The cell lines CEM, HPB-ALL, HSB2, Jurkat, MOLT4, Loucy, and PEER were obtained from either the American Type Culture Collection (ATCC; Manassas, VA, USA) or the DSMZ (German Collection of Microorganisms and Cell Culture; Braunschweig, Germany). The Jurkat-luciferase cell line has been previously described [28 (link)]. The Loucy-luciferase cell line was generously provided by Dr. Pieter Van Vlierberghe (Center for Medical Sciences, Ghent University, Ghent, Belgium). Short tandem repeat microsatellite loci analysis was used to confirm cell line identities. Cultures were confirmed Mycoplasma-free. Frozen cell line stocks were generated at the time of authentication and thawed for experiments ≤ 3 months before use. All cell lines were cultured in RPMI medium (Gibco, ThermoFisher Scientific, Waltham, MA, USA) supplemented with 10–20% fetal bovine serum (FBS) and 1% penicillin–streptomycin (complete RPMI (cRPMI)).
Summers R.J., Jain J., Vasileiadi E., Smith B., Chimenti M.L., Yeung T.Y., Kelvin J., Wang X., Frye S.V., Earp H.S., Tyner J.W., Dreaden E.C., DeRyckere D, & Graham D.K. (2022). Therapeutic Targeting of MERTK and BCL-2 in T-Cell and Early T-Precursor Acute Lymphoblastic Leukemia. Cancers, 14(24), 6142.
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8

Cultivation of T-ALL Cell Lines

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Human T-ALL cell lines (HPB-ALL, TALL-1, ALL-SIL, CUTLL1) and peripheral blood mononuclear cells (PBMC) as control were both procured from the American Type Culture Collection (ATCC; Rockville, MD), cultivated in RPMI-1640 medium (Gibco, Grand Island, NY) at 37°C in 95% air/5% CO2. Approximately 10% fetal bovine blood (FBS; Gibco) was employed for cell culture.
Li S., Guo W., Geng H., Wang C., Yang S, & Xu X. (2020). LINC00511 exacerbated T-cell acute lymphoblastic leukemia via miR-195-5p/LRRK1 axis. Bioscience Reports, 40(5), BSR20193631.
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9

Culturing Human T-ALL Cell Lines

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Human T-ALL cell lines (HPB-ALL, TALL-1, KOPTK1, Jurkat, CCRF-CEM, and Molt 4) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). Molt 4-luciferase (Molt 4-luc) cells with stable luciferase expression were obtained from PerkinElmer (Santa Clara, CA, USA). T-ALL cells were cultured in Roswell Park Memorial Institute-1640 (RPMI-1640; Gibco, BRL, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS; Gibco). All cells were incubated in a humidified incubator with 5% CO2 at 37°C. Cells from passages 2–4 were used for experiments.
Yang X.Y, & Sheng Y. (2019). miR-101 Represses T-Cell Acute Lymphoblastic Leukemia by Targeting CXCR7/STAT3 Axis. Oncology Research, 27(9), 997-1006.
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10

Culturing Diverse T-ALL Cell Lines and Engineered Ba/F3 Cells

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All human T-ALL cell lines (KOPT-K1, DU.528, HPB-ALL and SKW-3) were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA) or Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ; Braunschweig, Germany). Ba/F3 derivatives expressing various oncogenic fusion kinases, namely, TEL-ABL (ETV6-ABL1), TEL-JAK1 (ETV6-JAK1), TEL-JAK2 (ETV6-JAK2), and TEL-JAK3 (ETV6-JAK3), were obtained from Dr. Richard Morrigl and were described previously (Lacronique, et al 2000 (link)). Ba/F3 cells transformed by TEL-TYK2 were generated with the pBabe-Neo retroviral vector expressing ETV6 (TEL)-TYK2 cDNA (Lacronique, et al 2000 (link)), which was obtained from Dr. Olivier A. Bernard. These cells were maintained in RPMI-1640 medium (GIBCO, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO, USA) and 1% penicillin/streptomycin (Invitrogen, Waltham, MA, USA).
Akahane K., Li Z., Etchin J., Berezovskaya A., Gjini E., Masse C.E., Miao W., Rocnik J., Kapeller R., Greenwood J.R., Tiv H., Sanda T., Weinstock D.M, & Look A.T. (2017). Anti-leukaemic Activity of the TYK2 selective inhibitor NDI-031301 in T-cell Acute Lymphoblastic Leukaemia. British journal of haematology, 177(2), 271-282.
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