Human gastrointestinal carcinoid cells (BON), were gifted by Drs. Courtney M. Townsend, Jr. of the University of Texas Medical Branch (Galveston, TX, USA) and B. Mark Evers of the University of Kentucky (Lexington, KY, USA). Human bronchopulmonary carcinoid (H727) cells were purchased from the American Type Culture Collection (Manassas, VA, USA). BON and H727 cells were grown in DMEM/F-12 (Life Technologies, Grand Island, NY, USA) and RPMI/F-12 (Life Technologies, Grand Island, NY, USA), respectively, at a 5% CO2 and 37°C atmosphere. Media was supplemented with 100 IU/mL penicillin, 100µg/mL streptomycin (Life Technologies, Grand Island, NY, USA) and 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO, USA). ABT-888 (Selleck Chemicals, Houston, TX, USA) and dacarbazine (Sigma-Aldrich) were stored in aliquots of 10mM in DMSO at -80°C, and freshly thawed before use. Cells were plated at sub-confluency the day prior to treatment, and then incubated in fresh medium containing ABT-888 (0–10µM) for 24 hours, after which dacarbazine was added (0–1000µM) for 2 additional days. DMSO concentrations were normalized across all treatment groups.
H727 cells
Manufactured by American Type Culture Collection
Sourced in United States
H727) cells are a type of cell line maintained and distributed by American Type Culture Collection (ATCC). They are derived from human lung carcinoma tissue. H727) cells are commonly used in research applications, but a detailed description of their core function is not available while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Merck Group (3 mentions)
Penicillin, by Thermo Fisher Scientific (3 mentions)
Streptomycin, by Thermo Fisher Scientific (3 mentions)
Dacarbazine, by Merck Group (2 mentions)
Abt 888, by Selleck Chemicals (2 mentions)
Rpmi f 12, by Thermo Fisher Scientific (2 mentions)
Dmem f12, by Thermo Fisher Scientific (2 mentions)
Ham s f 12 k 1 1, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Dmem f12 medium, by Thermo Fisher Scientific (1 mentions)
3 protocols using h727 cells
1
Synergistic Effects of ABT-888 and Dacarbazine on Carcinoid Cells
2
Synergistic Effects of ABT-888 and Dacarbazine on Carcinoid Cells
3
Cultivation of Carcinoid Cell Lines
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BON cells, a human gastrointestinal carcinoid cell line, were obtained from Drs. B. Mark Evers and Courtney M. Townsend, Jr. (University of Texas Medical Branch, Galveston, TX) and were maintained in Dulbecco's modified Eagle medium‐nutrient mixture Ham's F‐12 K 1:1 (Invitrogen, Carlsbad, CA). H727 cells, a human bronchopulmonary carcinoid cell line, were purchased from American Type Culture Collection (Manassas, VA) and were maintained in RPMI1640 (Life Technologies, Grand Island, NY). MZ‐CRC‐1 cells, a human medullary thyroid cancer cell line, were provided by Dr. Barry Nelkin (Johns Hopkins, Baltimore, MD) and were maintained in DMEM/F‐12 medium (Life Technologies). All media were supplemented with 10% fetal bovine serum (Sigma‐Aldrich, St Louis, MO), 100 IU/mL penicillin (Invitrogen), and 100 μg/mL streptomycin (Invitrogen). All cell lines were grown in a humidified atmosphere of 5% CO2 at 37°C.
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