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3 protocols using 2 propanol

1

Polymer-based Microfluidic Device Fabrication

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Aniline was purchased from J&K Chemical (Hong Kong, China) and used without further purification. Ammonium peroxodisulfate (APS) and perfluorodecyltrichlorosilane (FDTS) were obtained from Alfa Aesar (Lancashire, United Kingdom). Hydrochloric acid was obtained from VWR (Batavia, USA). The COC film (Grade 8007) was commercially available from TOPAS Advanced Polymer (Frankfurt, Germany). Indium tin oxide (ITO) glass was obtained from South China Xiang Science & Technology (Shenzhen, China). Cu sulfate pentahydrate, sulfuric acid, and sodium dodecyl sulfate were purchased from Acros Organics (Geel, Belgium). Ethanol, 2-propanol, acetone, acetic acid, formaldehyde, toluene, and sodium bicarbonate were purchased from J&K Chemical (Hong Kong, China).
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2

Dopamine Hydrochloride Purification Protocol

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Dopamine hydrochloride (DA) was purchased from Acros Organics (New Jersey, USA). Ammonia aqueous solution, methanol, ethanol, and 2-propanol were obtained from J&K Scientific Ltd (Shanghai, China). Other chemical reagents were of analytical grade and were obtained commercially. All the solutions were prepared with Milli-Q purified water (Millipore, ≥18.2 MΩ cm).
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3

Cloning and Expression of iolS Gene

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The primers of iolS were synthesized at Comate Bioscience Co. Ltd (Changchun, China). Restriction enzyme DpnI and the primer STAR DNA polymerase were purchased from Takara Bio Inc. (Tokyo, Japan). High-performance liquid chromatography (HPLC)–grade hexane and 2-propanol were purchased from J&K Scientific Ltd. (Beijing, China). Recombinant E. coli Rosseta strains harboring pET28a-iolS and pET21a-GDH had been previously constructed in the laboratory [29 (link)].
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