Soybean lecithin (SPC), cholesterol, and DSPE-PEG2000-OMe were purchased from Aiweituo Biotech Co., Ltd. (Shanghai, China). DSPE-PEG2000-Mal was purchased from Avanti Polar Lipids (Birmingham, AL, USA). AUNP-12-GPLGVRGD-Cys (pep1), AUNP-12-GplgvRGD-Cys (pep2), and AUNP-12-GPLGV-Cys (pep3) were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China). NLG919 was gained from Dalian Meilun Biotech Co., Ltd. (Dalian, China). Anti-mouse PD-L1 antibody (ab213480), antimouse CD34 (ab81289) antibody, and rhMMP-2 (ab125181) were obtained from Abcam Ltd. (Shanghai, China). PE-anti-mouse CD25 antibody (101,904) and APC-anti-mouse CD40 antibody (124,612) were purchased from Biolegend (San Diego, CA, USA). DiD was purchased from Solarbio (Beijing, China). Cy3-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) (SA00009-2) was obtained from Proteintech (Rosemont, IL, USA). Murine breast cancer cells line (4T1), mouse mononuclear macrophage leukemia cells (RAW264.7), mouse T lymphocytes (CTLL-2), and mouse dendritic cells (DC2.4) were obtained from the Chinese Academy of Science cells Bank (Shanghai, China). Female BALB/c mice (18–20 g) were obtained from Chengdu ENSIWEIER Biotech Co., Ltd (Chengdu, China). All animal experiments were approved by the Animal Experimentation Ethics Committee of Sichuan University.
Cy3 conjugated affinipure goat anti rabbit igg h l sa00009 2
Manufactured by Proteintech
Sourced in United States
The Cy3-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) (SA00009-2) is a secondary antibody that can be used to detect and visualize rabbit primary antibodies in various applications. The antibody is conjugated with the Cy3 fluorescent dye, which allows for detection and visualization of the target using fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
Dspe peg2000 mal, by Avanti Polar Lipids (1 mentions)
N ethylmaleimide e3876, by Merck Group (1 mentions)
Gapdh gtx100118, by GeneTex (1 mentions)
Gtx133314, by GeneTex (1 mentions)
Ha h6908, by Merck Group (1 mentions)
Normal goat serum (ngs), by Beyotime (1 mentions)
Confocal microscope, by Zeiss (1 mentions)
P0131, by Beyotime (1 mentions)
A1 confocal microscopy, by Nikon (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions)
4 protocols using cy3 conjugated affinipure goat anti rabbit igg h l sa00009 2
1
Multifunctional Nanoplatform for Cancer Immunotherapy
2
Comprehensive ZIKV Protein Detection Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibodies against Flag (F3165, 1:2000) and HA (H6908, 1:5000) were purchased from Sigma (Burlington, MA, USA). Antibodies against the envelope protein of ZIKV (GTX133314, 1:1000), NS1 protein of ZIKV (GTX133307, 1:1000), glyceraldehyde-3-phosphate dehydrogenase (GAPDH; GTX100118, 1:5000), and ZDHHC11 (GTX106800, 1:1000) were purchased from GeneTex (Hsinchu City, Taiwan, P.R.C). For the immunofluorescence studies, antibodies against the envelope protein of flaviviruses (ab214333, 1:100) were purchased from Abcam (Shanghai, China). N-ethylmaleimide (E3876), hydroxylamine hydrochloride (255580), and 2-bromopalmitate (2-BP; 21604) were obtained from Sigma (Burlington, MA, USA). BMCC-biotin (21900) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Streptavidin-horseradish peroxidase (M00091) was purchased from Genscript (Piscataway, NJ, USA). Fluorescein (FITC)-conjugated AffiniPure goat anti-mouse immunoglobulin (Ig)G (H+L) (SA00003-1) and Cy3-conjugated AffiniPure goat anti-rabbit IgG (H+L) (SA00009-2) were purchased from ProteinTech (Rosemont, IL, USA).
3
Immunofluorescence Analysis of PGC-1α and GRP78 in A549 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The expression of PGC-1α and GRP78 in A549 cells was detected using immunofluorescence. Coverslips bearing A549 cells were collected from each group. The cells were fixed and permeabilized with 4% paraformaldehyde and 0.5% Triton X-100. Cells were blocked with 1% normal goat serum (Beyotime Biotechnology, Shanghai, China) for 30 min at room temperature and then incubated with primary antibodies PGC-1α (1 : 400) and GRP78 (1 : 200) overnight at 4 °C. Cy3-conjugated Affinipure Goat anti-rabbit IgG (H + L) (SA00009-2, Proteintech, Wuhan, China) was used as a secondary antibody and incubated for 1 h at room temperature in the dark. The cells were then fixed with an antifade mounting medium containing DAPI (P0131, Beyotime Biotechnology, Shanghai, China). Photomicrographs were obtained using a confocal microscope (ZEISS, Oberkochen, Germany).
4
Cardiomyocyte Hypertrophy Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
H9C2 was seeded in 24-well plates with a density of 2 × 10 3 cells per well for 12 h, then the media were replaced with Ang II (1 μM) containing media for 48 h in the absence/presence of CS extract. The H9C2 cells were fixed with 4% paraformaldehyde (PFA) for 10 min, washed with PBS three times, blocked with goat serum containing 1% Tween 20 for 1 h at room temperature, and then incubated with primary antibody Cardiac Troponin I (cTnI) (15513-1-AP, Proteintech, Rosemont, IL, USA) at 4 °C overnight. The secondary antibody of Cy3-conjugated Affinipure goat anti-rabbit IgG (H + L) (SA00009-2, Proteintech) was added and incubated in the dark at room temperature for another 1 h. Cell nuclear was stained with ready-to-use 4′6-diamidino-2-phenylindole (DAPI, Solarbio, C0065, Beijing, China) for 10 min. The images of H9C2 cells were detected by a Nikon A1 confocal microscopy (Nikon, Tokyo, Japan), and analyzed by the NIS-Elements Viewer software (version 5.21.00). The surface area of cells was calculated using the Image J software (National Institutes of Health, Bethesda, MD, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!